CBL Recombinant Rabbit Monoclonal Antibody [PSH17-61] - BSA and Azide free
Catalog# HA751635
CBL Recombinant Rabbit Monoclonal Antibody [PSH17-61] - BSA and Azide free
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WB
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IF-Cell
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FC
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IP
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Human
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Mouse
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Rat
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Green monkey
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HA723936
含抗保成分
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unconjugated
Overview
Product Name
CBL Recombinant Rabbit Monoclonal Antibody [PSH17-61] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CBL aa 441-840.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell, FC, IP
Molecular Weight
Predicted band size: 100 kDa
Positive Control
Raji cell lysate, K-562 cell lysate, COS-1 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Raji.
Conjugation
unconjugated
Clone Number
PSH17-61
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Cbl (named after Casitas B-lineage Lymphoma) is a mammalian gene family. CBL gene, a part of the Cbl family, encodes the protein CBL which is an E3 ubiquitin-protein ligase involved in cell signalling and protein ubiquitination. Mutations to this gene have been implicated in a number of human cancers, particularly acute myeloid leukaemia. Ubiquitination is the process of chemically attaching ubiquitin monomers to a protein, thereby targeting it for degradation. As this is a multi-step process, several different enzymes are involved, the final one being a member of the E3 family of ligases. Cbl functions as an E3 ligase, and therefore is able to catalyse the formation of a covalent bond between ubiquitin and Cbl's protein substrate - typically a receptor tyrosine kinase. The RING-finger domain mediates this transfer, however like other E3 ligases of the RING type no intermediate covalent bond is formed between ubiquitin and the RING-finger domain. The stepwise attachment of ubiquitin to the substrate receptor tyrosine kinase can lead to its removal from the plasma membrane and subsequent trafficking to the lysosome for degradation.
Background References
1. Leardini D et al. Role of CBL Mutations in Cancer and Non-Malignant Phenotype. Cancers (Basel). 2022 Feb
2. Pinilla-Macua I et al. Cbl and Cbl-b independently regulate EGFR through distinct receptor interaction modes. Mol Biol Cell. 2023 Dec
Subcellular Location
Cytoplasm, Cell membrane, Cell projection, cilium, Golgi apparatus.
Synonyms
4732447J05Rik antibody
C CBL antibody
Cas Br M (murine) ecotropic retroviral transforming sequence antibody
Casitas B lineage lymphoma proto oncogene antibody
Casitas B-lineage lymphoma proto-oncogene antibody
CBL 2 antibody
cbl antibody
CBL_HUMAN antibody
CBL2 antibody
E3 ubiquitin protein ligase CBL antibody
ExpandImages
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Western blot analysis of CBL on different lysates with Rabbit anti-CBL antibody (HA751635) at 1/5,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: COS-1 cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (30 µg/Lane)
Lane 5: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 100 kDa
Observed band size: 110 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751635) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Raji cells labeling CBL with Rabbit anti-CBL antibody (HA751635) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CBL antibody (HA751635) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of Raji cells labeling CBL.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751635, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
CBL was immunoprecipitated from 0.2 mg Raji cell lysate with HA751635 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751635 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate (input)
Lane 2: HA751635 IP in Raji cell lysate
Lane 3: Rabbit IgG instead of HA751635 in Raji cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CBL Recombinant Rabbit Monoclonal Antibody [PSH17-61]
Application: WB,IF-Cell,FC,IP
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
CBL Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
