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CD200 / OX2 Recombinant Rabbit Monoclonal Antibody [PSH17-62] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751636

CD200 / OX2 Recombinant Rabbit Monoclonal Antibody [PSH17-62] - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • WB

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD200 / OX2 Recombinant Rabbit Monoclonal Antibody [PSH17-62] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CD200 aa 1-232.

Species Reactivity

Mouse

Validated Applications

IHC-Fr, IHC-P, WB

Molecular Weight

Predicted band size: 31 kDa

Positive Control

Mouse brain tissue, mouse spleen tissue, Mouse brain tissue lysate, Mouse lung tissue lysate.

Conjugation

unconjugated

Clone Number

PSH17-62

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

  • WB

  • 1:5,000

Target

Function

OX-2 membrane glycoprotein, also named CD200 (Cluster of Differentiation 200) is a human protein encoded by the CD200 gene. In mice, the CD200 gene is located on chromosome 16. CD200 is expressed on dendritic cells, activated B lymphocytes, activated T lymphocytes, thymocytes, endothelial cells, neurons and osteoblast precursors. In innate immunity, cellular CD200 expression is induced upon TLR and NLR activation. At the transcriptional level, CD200 expression is regulated by C/EBP-β. It has been shown that CD200 expression is induced by IFN-γ and TNF-α in a NF-kappaB-, STAT1- and IRF-1-dependent manner.

Background References

1. Rauber S et al. CD200(+) fibroblasts form a pro-resolving mesenchymal network in arthritis. Nat Immunol. 2024 Apr

2. D\'Arena G et al. CD200 and Chronic Lymphocytic Leukemia: Biological and Clinical Relevance. Front Oncol. 2020 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: O54901 Mouse

Synonyms

Antigen identified by monoclonal antibody MRC OX 2 antibody

CD200 antibody

CD200 antigen antibody

CD200 molecule antibody

MOX1 antibody

MOX2 antibody

MRC antibody

MRC OX 2 antigen antibody

My033 antibody

OX 2 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CD200 / OX2 antibody (<a href="/products/HA751636" style="font-weight: bold;text-decoration: underline;">HA751636</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751636" style="font-weight: bold;text-decoration: underline;">HA751636</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CD200 / OX2 antibody (HA751636) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751636) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD200 / OX2 antibody (<a href="/products/HA751636" style="font-weight: bold;text-decoration: underline;">HA751636</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751636" style="font-weight: bold;text-decoration: underline;">HA751636</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD200 / OX2 antibody (HA751636) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751636) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD200 / OX2 on different lysates with Rabbit anti-CD200 / OX2 antibody (<a href="/products/HA751636" style="font-weight: bold;text-decoration: underline;">HA751636</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse lung tissue lysate<br />Lane 3: Mouse liver tissue lysate (low expression)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 40-50 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751636" style="font-weight: bold;text-decoration: underline;">HA751636</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD200 / OX2 on different lysates with Rabbit anti-CD200 / OX2 antibody (HA751636) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse lung tissue lysate
    Lane 3: Mouse liver tissue lysate (low expression)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 40-50 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751636) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"