intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-82] - BSA and Azide free
Catalog# HA751641
intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-82] - BSA and Azide free
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-82] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ALPI aa 1-503.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
Saos-2 cell lysate, Mouse small intestine tissue lysate.
Conjugation
unconjugated
Clone Number
PSH17-82
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Alkaline phosphatase, intestinal, also known as ALPI, is a type of alkaline phosphatase that in humans is encoded by the ALPI gene. Intestinal alkaline phosphatase is an endogenous enzyme that maintains gut homeostasis. It detoxifyies bacterial toxins, dephosphorylates phosphorylated nucleotides, regulates lipid absorption in the intestine, and regulates the microbiome in the intestine. In addition to these functions, intestinal alkaline phosphatase can also modulate bicarbonate secretion and can modulate the pH of the duodenum.
Background References
1. Santos GM et al. Intestinal Alkaline Phosphatase: A Review of This Enzyme Role in the Intestinal Barrier Function. Microorganisms. 2022 Mar
2. Singh SB et al. Role of Intestinal Alkaline Phosphatase in Innate Immunity. Biomolecules. 2021 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Alkaline phosphatase intestinal antibody
Alkaline phosphomonoesterase antibody
Alpi antibody
Glycerophosphatase antibody
IAP antibody
Intestinal alkaline phosphatase antibody
Intestinal-type alkaline phosphatase antibody
Kasahara isozyme antibody
OTTHUMP00000164357 antibody
PPBI_HUMAN antibody
Images
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Western blot analysis of intestinal alkaline phosphatase on different lysates with Rabbit anti-intestinal alkaline phosphatase antibody (HA751641) at 1/5,000 dilution.
Lane 1: Saos-2 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse small intestine tissue lysate (30 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 80 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751641) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"