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intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-83] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751642

intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-83] - BSA and Azide free

Application

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-83] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ALPI aa 1-503.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

Human small intestine tissue, human placenta tissue, mouse small intestine tissue, rat small intestine tissue.

Conjugation

unconjugated

Clone Number

PSH17-83

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000-1:30,000

Target

Function

Alkaline phosphatase, intestinal, also known as ALPI, is a type of alkaline phosphatase that in humans is encoded by the ALPI gene. Intestinal alkaline phosphatase is an endogenous enzyme that maintains gut homeostasis. It detoxifyies bacterial toxins, dephosphorylates phosphorylated nucleotides, regulates lipid absorption in the intestine, and regulates the microbiome in the intestine. In addition to these functions, intestinal alkaline phosphatase can also modulate bicarbonate secretion and can modulate the pH of the duodenum.

Background References

1. Santos GM et al. Intestinal Alkaline Phosphatase: A Review of This Enzyme Role in the Intestinal Barrier Function. Microorganisms. 2022 Mar

2. Singh SB et al. Role of Intestinal Alkaline Phosphatase in Innate Immunity. Biomolecules. 2021 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P09923 Human

SwissProt: P24822 Mouse

SwissProt: P15693 Rat

Synonyms

Alkaline phosphatase intestinal antibody

Alkaline phosphomonoesterase antibody

Alpi antibody

Glycerophosphatase antibody

IAP antibody

Intestinal alkaline phosphatase antibody

Intestinal-type alkaline phosphatase antibody

Kasahara isozyme antibody

OTTHUMP00000164357 antibody

PPBI_HUMAN antibody

Images

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA751642) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751642) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-intestinal alkaline phosphatase antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA751642) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751642) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/30,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA751642) at 1/30,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751642) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/30,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751642" style="font-weight: bold;text-decoration: underline;">HA751642</a>) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA751642) at 1/30,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751642) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"