Skip to content

ABCA7 Recombinant Rabbit Monoclonal Antibody [PSH18-11] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA751657

ABCA7 Recombinant Rabbit Monoclonal Antibody [PSH18-11] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ABCA7 Recombinant Rabbit Monoclonal Antibody [PSH18-11] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse ABCA7 aa 60-432.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 234 kDa

Positive Control

Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum (P7) tissue lysate, mouse thymus tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH18-11

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200

Target

Function

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Transports preferentially phosphatidylserine over phosphatidylcholine 2. Plays a role in lipid homeostasis and macrophage-mediated phagocytosis. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells 7, 8, 9. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocyte differentiation.Involved in lipid raft organization and CD1D localization on thymocytes and antigen-presenting cells, which plays an important role in natural killer T-cell development and activation (By similarity). Plays a role in phagocytosis of apoptotic cells by macrophages (By similarity). Macrophage phagocytosis is stimulated by APOA1 or APOA2, probably by stabilization of ABCA7 (By similarity). Also involved in phagocytic clearance of amyloid-beta by microglia cells and macrophages (By similarity). Further limits amyloid-beta production by playing a role in the regulation of amyloid-beta A4 precursor protein (APP) endocytosis and/or processing. Amyloid-beta is the main component of amyloid plaques found in the brains of Alzheimer patients.

Background References

1. Quazi F, Molday RS. Differential phospholipid substrates and directional transport by ATP-binding cassette proteins ABCA1, ABCA7, and ABCA4 and disease-causing mutants. J Biol Chem. 2013 Nov

2. Satoh K, Abe-Dohmae S, Yokoyama S, et al, Fraser PE. ATP-binding cassette transporter A7 (ABCA7) loss of function alters Alzheimer amyloid processing. J Biol Chem. 2015 Oct

Subcellular Location

Cell membrane, Golgi apparatus membrane, Early endosome membrane, Cytoplasm, Cell projection, ruffle membrane, phagocytic cup.

UNIPROT

SwissProt: Q91V24 Mouse

SwissProt: Q7TNJ2 Rat

Synonyms

ABC transporter ABCA7 antibody

ABC transporter member 7 antibody

ABCA SSN antibody

ABCA-SSN antibody

ABCA7 antibody

ABCX antibody

ATP binding cassette sub family A (ABC1) member 7 antibody

ATP binding cassette sub family A member 7 antibody

ATP binding Cassette Transporter A7 antibody

ATP-binding cassette sub-family A member 7 antibody

Expand

Images

  • Western blot analysis of ABCA7 on different lysates with Rabbit anti-ABCA7 antibody (<a href="/products/HA751657" style="font-weight: bold;text-decoration: underline;">HA751657</a>) at 1/5,000 dilution.<br /><br />Lane 1: Neuro-2a cell lysate (no heat) (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (no heat) (40 µg/Lane)<br />Lane 3: Mouse cerebellum (P7) tissue lysate (40 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 234 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751657" style="font-weight: bold;text-decoration: underline;">HA751657</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ABCA7 on different lysates with Rabbit anti-ABCA7 antibody (HA751657) at 1/5,000 dilution.

    Lane 1: Neuro-2a cell lysate (no heat) (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (no heat) (40 µg/Lane)
    Lane 3: Mouse cerebellum (P7) tissue lysate (40 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 234 kDa
    Observed band size: 250 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751657) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-ABCA7 antibody (<a href="/products/HA751657" style="font-weight: bold;text-decoration: underline;">HA751657</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751657" style="font-weight: bold;text-decoration: underline;">HA751657</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-ABCA7 antibody (HA751657) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751657) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ABCA7 antibody (<a href="/products/HA751657" style="font-weight: bold;text-decoration: underline;">HA751657</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751657" style="font-weight: bold;text-decoration: underline;">HA751657</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ABCA7 antibody (HA751657) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751657) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"