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LYVE1 Recombinant Rabbit Monoclonal Antibody [PSH18-29] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751665

LYVE1 Recombinant Rabbit Monoclonal Antibody [PSH18-29] - BSA and Azide free

Application

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

LYVE1 Recombinant Rabbit Monoclonal Antibody [PSH18-29] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human LYVE1 aa 20-322.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Human colon tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

PSH18-29

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:20,000

  • IF-Tissue

  • 1:2,000

Target

Function

Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1), also known as extracellular link domain containing 1 (XLKD1) is a Link domain-containing hyaladherin, a protein capable of binding to hyaluronic acid (HA), homologous to CD44, the main HA receptor. In humans it is encoded by the LYVE1 gene. LYVE1 is a type I integral membrane glycoprotein. It acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. LYVE-1 is a cell surface receptor on lymphatic endothelial cells that can be used as a lymphatic endothelial cell marker, allowing for the isolation of these cells for experimental purposes. The physiological role for this receptor is still the subject of debate, but evolutionary conservation suggests an important role. Expression of LYVE1 not restricted to lymph vessels but is also observed in normal liver blood sinusoids, and embryonic blood vessels.

Background References

1. Yu X et al. Septal LYVE1(+) macrophages control adipocyte stem cell adipogenic potential. Science. 2025 Aug

2. Siret C et al. Deciphering the heterogeneity of the Lyve1(+) perivascular macrophages in the mouse brain. Nat Commun. 2022 Nov

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q9Y5Y7 Human

Synonyms

Cell surface retention sequence-binding protein 1 antibody

CRSBP 1 antibody

CRSBP-1 antibody

CRSBP1 antibody

extracellular link domain containing 1 antibody

extracellular link domain-containing 1 antibody

Extracellular link domain-containing protein 1 antibody

HAR antibody

Hyaluronic acid receptor antibody

Lymphatic endothelium specific hyaluronan receptor antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-LYVE1 antibody (<a href="/products/HA751665" style="font-weight: bold;text-decoration: underline;">HA751665</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751665" style="font-weight: bold;text-decoration: underline;">HA751665</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-LYVE1 antibody (HA751665) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751665) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LYVE1 antibody (<a href="/products/HA751665" style="font-weight: bold;text-decoration: underline;">HA751665</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751665" style="font-weight: bold;text-decoration: underline;">HA751665</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LYVE1 antibody (HA751665) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751665) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: colon<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/2,000

    Application: IF-Tissue

    Species: Human

    Site: colon

    Sample: Paraffin-embedded section

    Antibody concentration: 1/2,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"