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Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH18-57] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751681

Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH18-57] - BSA and Azide free

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH18-57] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Prolactin aa 1-226.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 25 kDa

Positive Control

Mouse pituitary tissue lysate, Rat pituitary tissue lysate, human pituitary tissue, human pituitary adenoma tissue, mouse pituitary tissue, rat pituitary tissue.

Conjugation

unconjugated

Clone Number

PSH18-57

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100,000

  • IF-Tissue

  • 1:500

  • IHC-P

  • 1:2,000-1:8,000

Target

Function

Prolactin (PRL), also known as lactotropin and mammotropin, is a protein best known for its role in enabling mammals to produce milk. It is influential in over 300 separate processes in various vertebrates, including humans. Prolactin is secreted from the pituitary gland in response to eating, mating, estrogen treatment, ovulation and nursing. It is secreted heavily in pulses in between these events. Prolactin plays an essential role in metabolism, regulation of the immune system and pancreatic development.

Background References

1. Chasseloup F et al. Prolactin: structure, receptors, and functions. Rev Endocr Metab Disord. 2024 Dec

2. Rana M et al. Prolactin and its significance in the placenta. Hormones (Athens). 2022 Jun

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01236 Human

SwissProt: P06879 Mouse

SwissProt: P01237 Rat

Synonyms

Decidual prolactin antibody

GHA1 antibody

Growth hormone A1 antibody

Lactogenic hormone antibody

Luteotropic hormone antibody

Mammotropin antibody

PRL antibody

Prolactin antibody

Prolactin precursor antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Prolactin / PRL on different lysates with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/100,000 dilution.<br /><br />Lane 1: Mouse pituitary tissue lysate<br />Lane 2: Mouse kidney tissue lysate (negative)<br />Lane 3: Mouse heart tissue lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 23 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/100,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Prolactin / PRL on different lysates with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/100,000 dilution.

    Lane 1: Mouse pituitary tissue lysate
    Lane 2: Mouse kidney tissue lysate (negative)
    Lane 3: Mouse heart tissue lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 23 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751681) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Prolactin / PRL on different lysates with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/100,000 dilution.<br /><br />Lane 1: Rat pituitary tissue lysate<br />Lane 2: Rat kidney tissue lysate (negative)<br />Lane 3: Rat heart tissue lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 23 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/100,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Prolactin / PRL on different lysates with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/100,000 dilution.

    Lane 1: Rat pituitary tissue lysate
    Lane 2: Rat kidney tissue lysate (negative)
    Lane 3: Rat heart tissue lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 23 kDa

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751681) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: pituitary<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Mouse

    Site: pituitary

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Application: IF-Tissue<br /><br />Species: Rat<br /><br />Site: pituitary<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Rat

    Site: pituitary

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Immunohistochemical analysis of paraffin-embedded human pituitary tissue with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751681) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751681) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751681) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751681) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751681) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751681) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751681" style="font-weight: bold;text-decoration: underline;">HA751681</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (HA751681) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751681) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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