Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH19-33]
Catalog# HA724065
Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH19-33]
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH19-33]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Prolactin aa 1-227.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
Human pituitary tissue, human pituitary adenoma tissue, mouse pituitary tissue, rat pituitary tissue.
Conjugation
unconjugated
Clone Number
PSH19-33
Reactivity Data
| IHC-P | |
|---|---|
| Human |
|
| Mouse | |
| Rat |
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
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1:2,000-1:8,000
Target
Function
Prolactin (PRL), also known as luteotropic hormone or luteotropin, is a protein that is best known for its role in enabling mammals, usually females, to produce milk. It is influential in over 300 separate processes in various vertebrates, including humans. Prolactin is secreted from the pituitary gland in response to eating, mating, estrogen treatment, ovulation and nursing. Prolactin is secreted in pulses in between these events. Prolactin plays an essential role in metabolism, regulation of the immune system and pancreatic development. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. It has important cell cycle-related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor, binding to cytokine-like receptors, it influences hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways. The hormone acts in endocrine, autocrine and paracrine manner through the prolactin receptor and a large number of cytokine receptors. Pituitary prolactin secretion is regulated by endocrine neurons in the hypothalamus.
Background References
1. Schuler LA et al. Prolactin: The Third Hormone in Breast Cancer. Front Endocrinol (Lausanne). 2022 Jun
2. Szewczyk AK et al. Prolactin and oxytocin: potential targets for migraine treatment. J Headache Pain. 2023 Mar
Subcellular Location
Secreted.
Synonyms
Decidual prolactin antibody
GHA1 antibody
Growth hormone A1 antibody
Lactogenic hormone antibody
Luteotropic hormone antibody
Mammotropin antibody
PRL antibody
Prolactin antibody
Prolactin precursor antibody
Images
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Immunohistochemical analysis of paraffin-embedded human pituitary tissue with Rabbit anti-Prolactin / PRL antibody (HA724065) at 1/8,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724065) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue with Rabbit anti-Prolactin / PRL antibody (HA724065) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724065) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (HA724065) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724065) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Prolactin / PRL antibody (HA724065) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724065) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (HA724065) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724065) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Prolactin / PRL antibody (HA724065) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724065) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue (negative) with Rabbit anti-Prolactin / PRL antibody (HA724065) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724065) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH18-57] - BSA and Azide free
Application: WB,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat
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Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH18-57]
Application: WB,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Prolactin / PRL Recombinant Rabbit Monoclonal Antibody [PSH19-33] - BSA and Azide free
Application: IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
