RIP Recombinant Rabbit Monoclonal Antibody [PSH19-57] - BSA and Azide free
Catalog# HA751721
RIP Recombinant Rabbit Monoclonal Antibody [PSH19-57] - BSA and Azide free
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WB
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Human
-
Mouse
-
Rat
-
Green monkey
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unconjugated
Overview
Product Name
RIP Recombinant Rabbit Monoclonal Antibody [PSH19-57] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 76 kDa
Positive Control
HT-29 cell lysate, HeLa cell lysate, Raji cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
PSH19-57
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) functions in a variety of cellular pathways related to both cell survival and death. In terms of cell death, RIPK1 plays a role in apoptosis, necroptosis, and PANoptosis. Some of the cell survival pathways RIPK1 participates in include NF-κB, Akt, and JNK. RIPK1 is an enzyme that in humans is encoded by the RIPK1 gene, which is located on chromosome 6. This protein belongs to the Receptor Interacting Protein (RIP) kinases family, which consists of seven members, RIPK1 being the first member of the family.
Background References
1. Mannion J et al. A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death. Immunity. 2024 Jul
2. Hou S et al. PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis. Mol Cell. 2024 Mar
Subcellular Location
Cytoplasm, Cell membrane.
Synonyms
Cell death protein RIP antibody
FLJ39204 antibody
OTTHUMP00000039163 antibody
Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
receptor interacting protein 1 antibody
Receptor interacting protein antibody
Receptor interacting protein kinase 1 antibody
Receptor interacting serine threonine protein kinase 1 antibody
Receptor TNFRSF interacting serine threonine kinase 1 antibody
Receptor-interacting protein 1 antibody
ExpandImages
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Western blot analysis of RIP on different lysates with Rabbit anti-RIP antibody (HA751721) at 1/5,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Raji cell lysate
Lane 4: COS-1 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: MEF cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 75 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751721) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
RIP Recombinant Rabbit Monoclonal Antibody [PSH19-57]
Application: WB
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
