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VEGFR3 / FLT4 Recombinant Rabbit Monoclonal Antibody [PSH20-96] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751778

VEGFR3 / FLT4 Recombinant Rabbit Monoclonal Antibody [PSH20-96] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

VEGFR3 / FLT4 Recombinant Rabbit Monoclonal Antibody [PSH20-96] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse VEGFR3 / FLT4 aa 1-796.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 153 kDa

Positive Control

A549 cell lysate, mouse embryo tissue, rat embryo tissue.

Conjugation

unconjugated

Clone Number

PSH20-96

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Fms-related tyrosine kinase 4, also known as FLT4, is a protein which in humans is encoded by the FLT4 gene. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.

Background References

1. Ma L et al. FLT4/VEGFR3 activates AMPK to coordinate glycometabolic reprogramming with autophagy and inflammasome activation for bacterial elimination. Autophagy. 2022 Jun

2. Monaghan RM et al. FLT4 causes developmental disorders of the cardiovascular and lymphovascular systems via pleiotropic molecular mechanisms. Cardiovasc Res. 2024 Sep

Subcellular Location

Cell membrane, Cytoplasm, Nucleus.

UNIPROT

SwissProt: P35916 Human

SwissProt: P35917 Mouse

SwissProt: Q91ZT1 Rat

Synonyms

EC 2.7.10.1 antibody

flt 4 antibody

FLT-4 antibody

FLT4 antibody

FLT41 antibody

Fms related tyrosine kinase 4 antibody

Fms-like tyrosine kinase 4 antibody

LMPH1A antibody

PCL antibody

Soluble VEGFR3 variant 1 antibody

Expand

Images

  • Western blot analysis of VEGFR3 / FLT4 on different lysates with Rabbit anti-VEGFR3 / FLT4 antibody (<a href="/products/HA751778" style="font-weight: bold;text-decoration: underline;">HA751778</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: A549 treated with 10ng/mL TNF alpha and 10nM TPA for 24 hours cell lysate (20 µg/Lane)<br /><br />Predicted band size: 153 kDa<br />Observed band size: 180 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751778" style="font-weight: bold;text-decoration: underline;">HA751778</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VEGFR3 / FLT4 on different lysates with Rabbit anti-VEGFR3 / FLT4 antibody (HA751778) at 1/5,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: A549 treated with 10ng/mL TNF alpha and 10nM TPA for 24 hours cell lysate (20 µg/Lane)

    Predicted band size: 153 kDa
    Observed band size: 180 kDa

    Exposure time: 59 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751778) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-VEGFR3 / FLT4 antibody (<a href="/products/HA751778" style="font-weight: bold;text-decoration: underline;">HA751778</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751778" style="font-weight: bold;text-decoration: underline;">HA751778</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-VEGFR3 / FLT4 antibody (HA751778) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751778) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-VEGFR3 / FLT4 antibody (<a href="/products/HA751778" style="font-weight: bold;text-decoration: underline;">HA751778</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751778" style="font-weight: bold;text-decoration: underline;">HA751778</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-VEGFR3 / FLT4 antibody (HA751778) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751778) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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