LDL Receptor Recombinant Rabbit Monoclonal Antibody [PSH20-99] - BSA and Azide free
Catalog# HA751781
LDL Receptor Recombinant Rabbit Monoclonal Antibody [PSH20-99] - BSA and Azide free
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WB
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IF-Cell
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Human
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Mouse
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Rat
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HA724194
含抗保成分
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unconjugated
Overview
Product Name
LDL Receptor Recombinant Rabbit Monoclonal Antibody [PSH20-99] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LDL Receptor aa 1-788.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 95 kDa
Positive Control
HEK-293 cell lysate, Huh7 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Huh7.
Conjugation
unconjugated
Clone Number
PSH20-99
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:200
Target
Function
LDL receptor mediates the endocytosis of cholesterol-rich LDL and thus maintains the plasma level of LDL. This occurs in all nucleated cells, but mainly in the liver which removes ~70% of LDL from the circulation. LDL receptors are clustered in clathrin-coated pits, and coated pits pinch off from the surface to form coated endocytic vesicles that carry LDL into the cell. After internalization, the receptors dissociate from their ligands when they are exposed to lower pH in endosomes. After dissociation, the receptor folds back on itself to obtain a closed conformation and recycles to the cell surface. The rapid recycling of LDL receptors provides an efficient mechanism for delivery of cholesterol to cells. It was also reported that by association with lipoprotein in the blood, viruses such as hepatitis C virus, Flaviviridae viruses and bovine viral diarrheal virus could enter cells indirectly via LDLR-mediated endocytosis. LDLR has been identified as the primary mode of entry for the Vesicular stomatitis virus in mice and humans. In addition, LDLR modulation is associated with early atherosclerosis-related lymphatic dysfunction. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface.
Background References
1. Xie Y et al. Aberrant oligodendroglial LDL receptor orchestrates demyelination in chronic cerebral ischemia. J Clin Invest. 2021 Jan
2. Shin D et al. PCSK9 stimulates Syk, PKCdelta, and NF-kappaB, leading to atherosclerosis progression independently of LDL receptor. Nat Commun. 2024 Mar
Subcellular Location
Cell membrane, Membrane, clathrin-coated pit, Golgi apparatus, Early endosome, Late endosome, Lysosome.
Synonyms
FH antibody
FHC antibody
LDL R antibody
LDL receptor antibody
LDLCQ2 antibody
Ldlr antibody
LDLR_HUMAN antibody
Low Density Lipoprotein Receptor antibody
Low density lipoprotein receptor class A domain containing protein 3 antibody
Low density lipoprotein receptor familial hypercholesterolemia antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of LDL Receptor on different lysates with Rabbit anti-LDL Receptor antibody (HA751781) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate (low expression) (20 µg/Lane)
Lane 2: Huh7 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (30 µg/Lane)
Lane 4: Rat liver tissue lysate (30 µg/Lane)
Predicted band size: 95 kDa
Observed band size: 150 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751781) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Huh7 cells labeling LDL Receptor with Rabbit anti-LDL Receptor antibody (HA751781) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LDL Receptor antibody (HA751781) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"