IgD Recombinant Rabbit Monoclonal Antibody [JE39-23] - BSA and Azide free
Catalog# HA751821
IgD Recombinant Rabbit Monoclonal Antibody [JE39-23] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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Human
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HA722433
含抗保成分
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unconjugated
Overview
Product Name
IgD Recombinant Rabbit Monoclonal Antibody [JE39-23] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human IgD aa 50-150.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 48 kDa
Positive Control
Human serum lysate, Human plasma lysate, human tonsil tissue, human B-cell lymphoma tissue.
Conjugation
unconjugated
Clone Number
JE39-23
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
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IF-Tissue
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1:50
Target
Function
Immunoglobulin D (IgD) is an antibody isotype that makes up about 1% of proteins in the plasma membranes of immature B-lymphocytes where it is usually co-expressed with another cell surface antibody called IgM. IgD is also produced in a secreted form that is found in very small amounts in blood serum, representing 0.25% of immunoglobulins in serum. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains.
Background References
1. Itoh N et al. The dual aspects of IgD in the development of tolerance and the pathogenesis of allergic diseases. Allergol Int. 2023 Apr
2. Dirks J et al. IgD shapes the pre-immune naive B cell compartment in humans. Front Immunol. 2023 Jan
Subcellular Location
Secreted; Cell membrane.
UNIPROT
Synonyms
Immunoglobulin heavy constant delta
Ig delta chain C region
Ig delta chain C region NIG-65
Ig delta chain C region WAH
IGHD
Images
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Western blot analysis of IgD on different lysates with Rabbit anti-IgD antibody (HA751821) at 1/1,000 dilution.
Lane 1: Human serum lysate (40 µg/Lane)
Lane 2: Human plasma lysate (40 µg/Lane)
Predicted band size: 48 kDa
Observed band size: 72 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IgD antibody (HA751821) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751821) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-IgD antibody (HA751821) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751821) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"