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IgD Recombinant Rabbit Monoclonal Antibody [JE39-23]

Usd: 385

Specification

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Catalog# HA722433

IgD Recombinant Rabbit Monoclonal Antibody [JE39-23]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IgD Recombinant Rabbit Monoclonal Antibody [JE39-23]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human IgD aa 50-150.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 48 kDa

Positive Control

Human serum lysate, Human plasma lysate, human tonsil tissue, human B-cell lymphoma tissue.

Conjugation

unconjugated

Clone Number

JE39-23

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:50

Target

Function

Immunoglobulin D (IgD) is an antibody isotype that makes up about 1% of proteins in the plasma membranes of immature B-lymphocytes where it is usually co-expressed with another cell surface antibody called IgM. IgD is also produced in a secreted form that is found in very small amounts in blood serum, representing 0.25% of immunoglobulins in serum. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains.

Background References

1. Itoh N et al. The dual aspects of IgD in the development of tolerance and the pathogenesis of allergic diseases. Allergol Int. 2023 Apr

2. Dirks J et al. IgD shapes the pre-immune naive B cell compartment in humans. Front Immunol. 2023 Jan

Subcellular Location

Secreted; Cell membrane.

UNIPROT

SwissProt: P01880 Human

Synonyms

Immunoglobulin heavy constant delta

Ig delta chain C region

Ig delta chain C region NIG-65

Ig delta chain C region WAH

IGHD

Images

  • Western blot analysis of IgD on different lysates with Rabbit anti-IgD antibody (<a href="/products/HA722433" style="font-weight: bold;text-decoration: underline;">HA722433</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human serum lysate (40 µg/Lane)<br />Lane 2: Human plasma lysate (40 µg/Lane)<br /><br />Predicted band size: 48 kDa<br />Observed band size: 72 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722433" style="font-weight: bold;text-decoration: underline;">HA722433</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IgD on different lysates with Rabbit anti-IgD antibody (HA722433) at 1/1,000 dilution.

    Lane 1: Human serum lysate (40 µg/Lane)
    Lane 2: Human plasma lysate (40 µg/Lane)

    Predicted band size: 48 kDa
    Observed band size: 72 kDa

    Exposure time: 25 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722433) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IgD antibody (<a href="/products/HA722433" style="font-weight: bold;text-decoration: underline;">HA722433</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722433" style="font-weight: bold;text-decoration: underline;">HA722433</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IgD antibody (HA722433) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722433) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-IgD antibody (<a href="/products/HA722433" style="font-weight: bold;text-decoration: underline;">HA722433</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722433" style="font-weight: bold;text-decoration: underline;">HA722433</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-IgD antibody (HA722433) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722433) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation