LI Cadherin Recombinant Rabbit Monoclonal Antibody [JE60-34] - BSA and Azide free

☑ Relative expression (RE)

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Western blot analysis of LI Cadherin on different lysates with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (30 µg/Lane)
Lane 3: PANC-1 cell lysate (negative) (30 µg/Lane)

Predicted band size: 92 kDa
Observed band size: 120 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721895) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Caco-2 cells labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721895) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of paraffin-embedded human appendix tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721895, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

This data was developed using HA721895, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Caco-2 cells labeling LI Cadherin.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721895, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).