LI Cadherin Recombinant Rabbit Monoclonal Antibody [JE60-34]
Catalog# HA721895
LI Cadherin Recombinant Rabbit Monoclonal Antibody [JE60-34]
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WB
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IHC-P
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IF-Tissue
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IF-Cell
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
LI Cadherin Recombinant Rabbit Monoclonal Antibody [JE60-34]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human LI Cadherin aa 1-100 / 832.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Tissue, IF-Cell, FC
Target Molecular Weight
Predicted band size: 92 kDa
Positive Control
Caco-2 cell lysate, LoVo cell lysate, Caco-2, human appendix tissue, human stomach tissue, mouse small intestine tissue.
Conjugation
unconjugated
Clone Number
JE60-34
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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IF-Tissue
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1:100
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Background References
1. Dantzig A.H., Hoskins J., Tabas L.B., Bright S., Shepard R.L., Jenkins I.L., Duckworth D.C., Sportsman J.R., MacKensen D. Association of intestinal peptide transport with a protein related to the cadherin superfamily. Science 264:430-433 (1994)
2. Yu Q., Shen W., Zhou H., Dong W., Gao D. Knockdown of LI-cadherin alters expression of matrix metalloproteinase-2 and -9 and galectin-3. Mol Med Rep 13:4469-4474 (2016)
Subcellular Location
Cell membrane
UNIPROT
Synonyms
BILL-cadherin antibody
CAD17_HUMAN antibody
Cadherin 16 antibody
Cadherin 16, formerly antibody
Cadherin 17 antibody
cadherin 17, LI cadherin (liver-intestine) antibody
cadherin, liver-intestine antibody
Cadherin-17 antibody
CDH16 antibody
CDH16, formerly antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of LI Cadherin on different lysates with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.
Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (30 µg/Lane)
Lane 3: PANC-1 cell lysate (negative) (30 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 120 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721895) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Caco-2 cells labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human appendix tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721895, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Flow cytometric analysis of Caco-2 cells labeling LI Cadherin.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721895, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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