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TRP2 / DCT Recombinant Rabbit Monoclonal Antibody [PSH22-31] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751859

TRP2 / DCT Recombinant Rabbit Monoclonal Antibody [PSH22-31] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TRP2 / DCT Recombinant Rabbit Monoclonal Antibody [PSH22-31] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TRP2 / DCT aa 1-250.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 59 kDa

Positive Control

MeWo cell lysate, SK-MEL-28 cell lysate.

Conjugation

unconjugated

Clone Number

PSH22-31

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

Dopachrome tautomerase (dopachrome delta-isomerase, tyrosinase-related protein 2), also known as DCT, is a human gene. Its expression is regulated by the microphthalmia-associated transcription factor (MITF).

Background References

1. Tingaud-Sequeira A et al. The Dct(-/-) Mouse Model to Unravel Retinogenesis Misregulation in Patients with Albinism. Genes (Basel). 2022 Jun

2. Volk AE et al. Biallelic mutations in L-dopachrome tautomerase (DCT) cause infantile nystagmus and oculocutaneous albinism. Hum Genet. 2021 Aug

Subcellular Location

Melanosome membrane, Melanosome.

UNIPROT

SwissProt: P40126 Human

Synonyms

DCT antibody

TYRP2 antibody

L-dopachrome tautomerase antibody

DCT antibody

DT antibody

L-dopachrome Delta-isomerase antibody

Tyrosinase-related protein 2 antibody

TRP-2 antibody

TRP2 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TRP2 / DCT on different lysates with Rabbit anti-TRP2 / DCT antibody (<a href="/products/HA751859" style="font-weight: bold;text-decoration: underline;">HA751859</a>) at 1/5,000 dilution.<br /><br />Lane 1: MeWo cell lysate (15 µg/Lane)<br />Lane 2: SK-MEL-28 cell lysate (15 µg/Lane)<br />Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane)<br /><br />Predicted band size: 59 kDa<br />Observed band size: 69/85 kDa<br /><br />Exposure time: 8 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751859" style="font-weight: bold;text-decoration: underline;">HA751859</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TRP2 / DCT on different lysates with Rabbit anti-TRP2 / DCT antibody (HA751859) at 1/5,000 dilution.

    Lane 1: MeWo cell lysate (15 µg/Lane)
    Lane 2: SK-MEL-28 cell lysate (15 µg/Lane)
    Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane)

    Predicted band size: 59 kDa
    Observed band size: 69/85 kDa

    Exposure time: 8 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751859) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • TRP2 / DCT was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with <a href="/products/HA751859" style="font-weight: bold;text-decoration: underline;">HA751859</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751859" style="font-weight: bold;text-decoration: underline;">HA751859</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: SK-MEL-28 cell lysate (input)<br />Lane 2: <a href="/products/HA751859" style="font-weight: bold;text-decoration: underline;">HA751859</a> IP in SK-MEL-28 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751859" style="font-weight: bold;text-decoration: underline;">HA751859</a> in SK-MEL-28 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 1 minute 2 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TRP2 / DCT was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA751859 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751859 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: SK-MEL-28 cell lysate (input)
    Lane 2: HA751859 IP in SK-MEL-28 cell lysate
    Lane 3: Rabbit IgG instead of HA751859 in SK-MEL-28 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 1 minute 2 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"