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Thioredoxin Recombinant Rabbit Monoclonal Antibody [PSH23-52] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751900

Thioredoxin Recombinant Rabbit Monoclonal Antibody [PSH23-52] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Thioredoxin Recombinant Rabbit Monoclonal Antibody [PSH23-52] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Thioredoxin aa 1-105.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 12 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cells) cell lysate, Hep G2 (Human liver cancer cells) cell lysate, MCF7 (Human breast cancer cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, Neuro-2a (Mouse brain neuroblastoma cells) cell lysate, C2C12 (Mouse myoblasts) cell lysate, C6 (Rat glioma cells) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

PSH23-52

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. TrxR is a ubiquitously expressed flavoprotein that catalyzes the NADPH-dependent reduction of Trx as well as several other oxidized cellular components.

Background References

1. Masutani H. Thioredoxin-Interacting Protein in Cancer and Diabetes. Antioxid Redox Signal. 2022 May

2. Jastrząb A et al. Thioredoxin-dependent system. Application of inhibitors. J Enzyme Inhib Med Chem. 2021 Dec

Subcellular Location

Nucleus, Cytoplasm, Secreted.

UNIPROT

SwissProt: P10599 Human

SwissProt: P10639 Mouse

SwissProt: P11232 Rat

Synonyms

ADF antibody

ATL derived factor antibody

ATL-derived factor antibody

DKFZp686B1993 antibody

MGC61975 antibody

SASP antibody

Surface associated sulphydryl protein antibody

Surface-associated sulphydryl protein antibody

testicular tissue protein Li 199 antibody

THIO_HUMAN antibody

Expand

Images

  • Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (<a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate <br />Lane 2: Hep G2 (Human liver cancer cells) cell lysate <br />Lane 3: MCF7 (Human breast cancer cells) cell lysate <br />Lane 4: A549 (Human lung adenocarcinoma cells) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 9 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 12 kDa <br />Observed band size: 12 kDa

    Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (HA751900) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
    Lane 2: Hep G2 (Human liver cancer cells) cell lysate
    Lane 3: MCF7 (Human breast cancer cells) cell lysate
    Lane 4: A549 (Human lung adenocarcinoma cells) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 9 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751900, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (<a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a>) at 1/5,000 dilution. <br /> <br />Lane 1: COS-1 (African green monkey kidney fibroblasts) cell lysate <br />Lane 2: NIH/3T3 (Mouse fibroblasts) cell lysate <br />Lane 3: Neuro-2a (Mouse brain neuroblastoma cells) cell lysate <br />Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate <br />Lane 5: C2C12 (Mouse myoblasts) cell lysate <br />Lane 6: C6 (Rat glioma cells) cell lysate <br />Lane 7: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate <br />Lane 8: Mouse kidney tissue lysate <br />Lane 9: Rat kidney tissue lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 9 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 12 kDa <br />Observed band size: 12 kDa

    Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (HA751900) at 1/5,000 dilution.

    Lane 1: COS-1 (African green monkey kidney fibroblasts) cell lysate
    Lane 2: NIH/3T3 (Mouse fibroblasts) cell lysate
    Lane 3: Neuro-2a (Mouse brain neuroblastoma cells) cell lysate
    Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate
    Lane 5: C2C12 (Mouse myoblasts) cell lysate
    Lane 6: C6 (Rat glioma cells) cell lysate
    Lane 7: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
    Lane 8: Mouse kidney tissue lysate
    Lane 9: Rat kidney tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 9 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751900, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Immunoprecipitation (IP) <br /> <br />Thioredoxin was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with <a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. <br /> <br />Lane 1: HeLa cell lysate (input) <br />Lane 2: <a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a> IP in HeLa cell lysate <br />Lane 3: Rabbit IgG instead of <a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a> in HeLa cell lysate <br /> <br />Exposure time: 2 seconds <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Primary dilution: <a href="/products/HA751900" style="font-weight: bold;text-decoration: underline;">HA751900</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature <br /> <br />Predicted band size: 12 kDa <br />Observed band size: 12 kDa

    Immunoprecipitation (IP)

    Thioredoxin was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA751900 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751900 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA751900 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA751900 in HeLa cell lysate

    Exposure time: 2 seconds
    Blocking: 5% NFDM/TBST, 1 hour at room temperature

    Primary dilution: HA751900, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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