NG2/CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH24-57] - BSA and Azide free
Catalog# HA751947
NG2/CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH24-57] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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FC
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
NG2/CSPG4 Recombinant Rabbit Monoclonal Antibody [PSH24-57] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse CSPG4 aa 151-600.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, IF-Tissue, FC
Target Molecular Weight
Predicted band size: 251 kDa
Positive Control
A375 (Human amelanotic melanoma cells) cell lysate, SK-MEL-28 (Human malignant melanoma cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, Mouse brain tissue lysate, Mouse spinal cord tissue lysate, Rat brain tissue lysate, Rat pancreas tissue lysate.
Conjugation
unconjugated
Clone Number
PSH24-57
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. CSPG4/NG2 is also a hallmark protein of oligodendrocyte progenitor cells (OPCs)[8] and OPC dysfunction has been implicated as a candidate pathophysiological mechanism of familial schizophrenia.
Background References
1. Greiner D et al. Human CSPG4-targeting CAR-macrophages inhibit melanoma growth. Oncogene. 2025 Jun
2. Chen X et al. Chondroitin Sulfate Proteoglycan 4 (CSPG4) as an Emerging Target for Immunotherapy to Treat Melanoma. Cancers (Basel). 2024 Sep
Subcellular Location
Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface.
Synonyms
4732461B14Rik antibody
AN2 antibody
AN2 proteoglycan antibody
Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
Chondroitin sulfate proteoglycan 4 antibody
Chondroitin sulfate proteoglycan NG2 antibody
Cspg4 antibody
Cspg4 chondroitin sulfate proteoglycan 4 antibody
CSPG4_HUMAN antibody
HMW-MAA antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Western blot analysis of NG2/CSPG4 on different lysates with Rabbit anti-NG2/CSPG4 antibody (HA724453) at 1/5,000 dilution.
Lane 1: A375 (Human amelanotic melanoma cells) cell lysate
Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 5: SK-BR-3 (Human breast cancer cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 9 seconds; ECL: K1801
MCF7 is a negative control (PMID: 20852124).
SK-BR-3 is a negative control (PMID: 20852124).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724453, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 251 kDa
Observed band size: 280,330 kDa -
☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Western blot analysis of NG2/CSPG4 on different lysates with Rabbit anti-NG2/CSPG4 antibody (HA724453) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Mouse spinal cord tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat liver tissue lysate
Lane 6: Rat pancreas tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Liver is a negative control (PMID: 23481707).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724453, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 251 kDa
Observed band size: 330 kDa -
☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724453, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Negative control: SK-Br-3 (PMID: 20852124). -
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Choroid plexus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724453, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724453, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724453, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Mouse liver tissue is a negative control (PMID: 23481707). -
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724453, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724453, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Rat liver tissue is a negative control (PMID: 23481707). -
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724453, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Liver is a negative control (PMID: 23481707).
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724453, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724453, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Liver
Sample: Paraffin-embedded section
Liver is a negative control (PMID: 23481707).
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724453, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724453, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry
Species: Human
Sample: A375 (Human amelanotic melanoma cells)/SK-BR-3 (Human breast cancer cells)
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724453 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: SK-Br-3 (PMID: 20852124).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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