PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71] - BSA and Azide free
Catalog# HA752013
PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PGC1 alpha aa 755-798 /798 and aa 269-323 /798.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 91/113 kDa
Positive Control
SK-Br-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Human kidney tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Mouse heart tissue lysate, Rat heart tissue lysate.
Conjugation
unconjugated
Clone Number
JF09-71
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is a protein that in humans is encoded by the PPARGC1A gene. PPARGC1A is also known as human accelerated region 20 (HAR20). It may, therefore, have played a key role in differentiating humans from apes. PGC-1α is the master regulator of mitochondrial biogenesis. PGC-1α is also the primary regulator of liver gluconeogenesis, inducing increased gene expression for gluconeogeneis. PGC-1α is a transcriptional coactivator that regulates the genes involved in energy metabolism. Peroxisome proliferator-activated receptor gamma coactivator 1-beta is a protein that in humans is encoded by the PPARGC1B gene. The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity.
Background References
1. Jiao J et al. Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet. Food Nutr Res 60:31304 (2016).
2. Al-Rewashdy H et al. Utrophin A is essential in mediating the functional adaptations of mdx mouse muscle following chronic AMPK activation. Hum Mol Genet 24:1243-55 (2015).
Tissue Specificity
Ubiquitous with higher expression in heart, brain and skeletal muscle.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
LEM6 antibody
Ligand effect modulator 6 antibody
PERC antibody
Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha antibody
Peroxisome proliferative activated receptor, gamma, coactivator 1 antibody
Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody
peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody
Peroxisome proliferator-activated receptor gamma coactivator 1-beta antibody
peroxisome proliferator-activated receptor gamma, coactivator 1 beta antibody
ExpandImages
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Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (HA752013) at 1/2,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: C6 cell lysate
Lane 4: Human kidney tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Rat kidney tissue lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Rat brain tissue lysate
Lane 9: Mouse heart tissue lysate
Lane 10: Rat heart tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 91/113 kDa
Observed band size: 91/113 kDa
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA752013) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (HA752013) at 1/1,000 dilution.
Lane 1: His-tagged PGC1 alpha recombinant protein
Lane 2: His-tagged PGC1 beta recombinant protein
Lysates/proteins at 50 ng/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA752013) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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