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PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71]

Usd: 385

Specification

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Catalog# ET1702-96

PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PGC1 alpha+beta Recombinant Rabbit Monoclonal Antibody [JF09-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PGC1 alpha aa 755-798 /798 and aa 269-323 /798.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 91/113 kDa

Positive Control

SK-Br-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Human kidney tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Mouse heart tissue lysate, Rat heart tissue lysate.

Conjugation

unconjugated

Clone Number

JF09-71

RRID

AB_3070355

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is a protein that in humans is encoded by the PPARGC1A gene. PPARGC1A is also known as human accelerated region 20 (HAR20). It may, therefore, have played a key role in differentiating humans from apes. PGC-1α is the master regulator of mitochondrial biogenesis. PGC-1α is also the primary regulator of liver gluconeogenesis, inducing increased gene expression for gluconeogeneis. PGC-1α is a transcriptional coactivator that regulates the genes involved in energy metabolism. Peroxisome proliferator-activated receptor gamma coactivator 1-beta is a protein that in humans is encoded by the PPARGC1B gene. The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity.

Background References

1. Jiao J et al. Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet. Food Nutr Res 60:31304 (2016).

2. Al-Rewashdy H et al. Utrophin A is essential in mediating the functional adaptations of mdx mouse muscle following chronic AMPK activation. Hum Mol Genet 24:1243-55 (2015).

Tissue Specificity

Ubiquitous with higher expression in heart, brain and skeletal muscle.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q86YN6 Human

SwissProt: Q9UBK2 Human

SwissProt: 415302 Mouse

SwissProt: O70343 Mouse

SwissProt: Q8VHJ7 Mouse

SwissProt: Q811R2 Rat

SwissProt: Q9QYK2 Rat

Synonyms

LEM6 antibody

Ligand effect modulator 6 antibody

PERC antibody

Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha antibody

Peroxisome proliferative activated receptor, gamma, coactivator 1 antibody

Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody

peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody

Peroxisome proliferator-activated receptor gamma coactivator 1-beta antibody

peroxisome proliferator-activated receptor gamma, coactivator 1 beta antibody

Expand

Images

  • Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (<a href="/products/ET1702-96" style="font-weight: bold;text-decoration: underline;">ET1702-96</a>) at 1/2,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: Human kidney tissue lysate<br />Lane 5: Mouse kidney tissue lysate<br />Lane 6: Rat kidney tissue lysate<br />Lane 7: Mouse brain tissue lysate<br />Lane 8: Rat brain tissue lysate<br />Lane 9: Mouse heart tissue lysate<br />Lane 10: Rat heart tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 91/113 kDa<br />Observed band size: 91/113 kDa<br /><br />Exposure time: 43 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-96" style="font-weight: bold;text-decoration: underline;">ET1702-96</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/2,000 dilution.

    Lane 1: SK-Br-3 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: C6 cell lysate
    Lane 4: Human kidney tissue lysate
    Lane 5: Mouse kidney tissue lysate
    Lane 6: Rat kidney tissue lysate
    Lane 7: Mouse brain tissue lysate
    Lane 8: Rat brain tissue lysate
    Lane 9: Mouse heart tissue lysate
    Lane 10: Rat heart tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 91/113 kDa
    Observed band size: 91/113 kDa

    Exposure time: 43 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (<a href="/products/ET1702-96" style="font-weight: bold;text-decoration: underline;">ET1702-96</a>) at 1/1,000 dilution.<br /><br />Lane 1: His-tagged PGC1 alpha recombinant protein<br />Lane 2: His-tagged PGC1 beta recombinant protein<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-96" style="font-weight: bold;text-decoration: underline;">ET1702-96</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PGC1 alpha+beta on different lysates with Rabbit anti-PGC1 alpha+beta antibody (ET1702-96) at 1/1,000 dilution.

    Lane 1: His-tagged PGC1 alpha recombinant protein
    Lane 2: His-tagged PGC1 beta recombinant protein

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-96) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation