SOD1 Recombinant Rabbit Monoclonal Antibody [PSH26-10] - BSA and Azide free
Catalog# HA752029
SOD1 Recombinant Rabbit Monoclonal Antibody [PSH26-10] - BSA and Azide free
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WB
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IF-Cell
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FC(Intra)
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Human
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Mouse
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Rat
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Pig
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unconjugated
Overview
Product Name
SOD1 Recombinant Rabbit Monoclonal Antibody [PSH26-10] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within rat SOD1 aa 1-154.
Species Reactivity
Human, Mouse, Rat, Pig
Validated Applications
WB, IF-Cell, FC(Intra)
Target Molecular Weight
Predicted band size: 16 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human liver cancer cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C2C12 (Mouse myoblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat stomach tissue lysate, Pig brain tissue lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, RAW264.7 treated with 100ng/mL LPS for 24 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH26-10
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:500
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FC(Intra)
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1:1,000
Target
Function
Cu-Zn superoxide dismutase-1 (SOD-1) is a well characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer's disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.
Background References
1. Greco T et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016).
2. Liebl MP et al. Low-frequency magnetic fields do not aggravate disease in mouse models of Alzheimer\'s disease and amyotrophic lateral sclerosis. Sci Rep 5:8585 (2015).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
Cu/Zn SOD antibody
Cu/Zn superoxide dismutase antibody
SOD1 antibody
Superoxide dismutase [Cu-Zn] antibody
Superoxide dismutase 1 antibody
Superoxide dismutase 1 soluble antibody
Superoxide dismutase Cu Zn antibody
Images
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Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA752029) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Hep G2 (Human liver cancer cell) cell lysate
Lane 3: HUVEC (Human umbilical vein endothelial cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752029, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 16 kDa
Observed band size: 16 kDa -
Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA752029) at 1/5,000 dilution.
Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lane 3: C2C12 (Mouse myoblast) cell lysate
Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752029, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 16 kDa
Observed band size: 16 kDa -
Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA752029) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat liver tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat stomach tissue lysate
Lane 6: Pig brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 4 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752029, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 16 kDa
Observed band size: 16 kDa -
☑ Cell treatment (CT)
Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA752029) at 1/5,000 dilution.
Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 2: RAW264.7 treated with 100ng/mL LPS for 24 hours cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752029, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 16 kDa
Observed band size: 16 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: Neuro-2a (Mouse brain neuroblastoma cell)
% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA752029, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: Neuro-2a (Mouse brain neuroblastoma cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA752029 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"