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SOD1 Recombinant Rabbit Monoclonal Antibody [PSH26-10]

Usd: 385

Specification

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Catalog# HA724615

SOD1 Recombinant Rabbit Monoclonal Antibody [PSH26-10]

Application

  • WB

  • IF-Cell

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

  • Pig

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

SOD1 Recombinant Rabbit Monoclonal Antibody [PSH26-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within rat SOD1 aa 1-154.

Species Reactivity

Human, Mouse, Rat, Pig

Validated Applications

WB, IF-Cell, FC(Intra)

Target Molecular Weight

Predicted band size: 16 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human liver cancer cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C2C12 (Mouse myoblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat stomach tissue lysate, Pig brain tissue lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, RAW264.7 treated with 100ng/mL LPS for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH26-10

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:500

  • FC(Intra)

  • 1:1,000

Target

Function

Cu-Zn superoxide dismutase-1 (SOD-1) is a well characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer's disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.

Background References

1. Greco T et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016).

2. Liebl MP et al. Low-frequency magnetic fields do not aggravate disease in mouse models of Alzheimer\'s disease and amyotrophic lateral sclerosis. Sci Rep 5:8585 (2015).

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P00441 Human

SwissProt: P08228 Mouse

SwissProt: P07632 Rat

Synonyms

Cu/Zn SOD antibody

Cu/Zn superoxide dismutase antibody

SOD1 antibody

Superoxide dismutase [Cu-Zn] antibody

Superoxide dismutase 1 antibody

Superoxide dismutase 1 soluble antibody

Superoxide dismutase Cu Zn antibody

Images

  • Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (<a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate <br />Lane 2: Hep G2 (Human liver cancer cell) cell lysate <br />Lane 3: HUVEC (Human umbilical vein endothelial cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 16 kDa <br />Observed band size: 16 kDa

    Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: Hep G2 (Human liver cancer cell) cell lysate
    Lane 3: HUVEC (Human umbilical vein endothelial cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (<a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>) at 1/5,000 dilution. <br /> <br />Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate <br />Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate <br />Lane 3: C2C12 (Mouse myoblast) cell lysate <br />Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 16 kDa <br />Observed band size: 16 kDa

    Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.

    Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
    Lane 3: C2C12 (Mouse myoblast) cell lysate
    Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (<a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>) at 1/5,000 dilution. <br /> <br />Lane 1: Mouse liver tissue lysate <br />Lane 2: Mouse brain tissue lysate <br />Lane 3: Rat liver tissue lysate <br />Lane 4: Rat brain tissue lysate <br />Lane 5: Rat stomach tissue lysate <br />Lane 6: Pig brain tissue lysate <br /> <br />Lysates/proteins at 30 µg/Lane. <br />Exposure time: 4 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 16 kDa <br />Observed band size: 16 kDa

    Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Rat liver tissue lysate
    Lane 4: Rat brain tissue lysate
    Lane 5: Rat stomach tissue lysate
    Lane 6: Pig brain tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 4 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (<a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>) at 1/5,000 dilution. <br /> <br />Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate <br />Lane 2: RAW264.7 treated with 100ng/mL LPS for 24 hours cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 16 kDa <br />Observed band size: 16 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of SOD1 on different lysates with Rabbit anti-SOD1 antibody (HA724615) at 1/5,000 dilution.

    Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
    Lane 2: RAW264.7 treated with 100ng/mL LPS for 24 hours cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724615, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Mouse <br />Sample: Neuro-2a (Mouse brain neuroblastoma cell) <br /> <br />% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: Neuro-2a (Mouse brain neuroblastoma cell)

    % Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724615, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • Application: Flow Cytometry (Intra)<br /><br />Species: Mouse<br />Sample: Neuro-2a (Mouse brain neuroblastoma cells)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/HA724615" style="font-weight: bold;text-decoration: underline;">HA724615</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Mouse
    Sample: Neuro-2a (Mouse brain neuroblastoma cells)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724615 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"