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CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752039

CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71] - BSA and Azide free

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 97 kDa

Positive Control

Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH26-71

Reactivity Data

WB IHC-P FC
Human
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

CD133 antigen, also known as prominin-1, is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins, which specifically localize to cellular protrusions. When embedded in the cell membrane, the membrane topology of prominin-1 is such that the N-terminus extends into the extracellular space and the C-terminus resides in the intracellular compartment. The protein consists of five transmembrane segments, with the first and second segments and the third and fourth segments connected by intracellular loops while the second and third as well as fourth and fifth transmembrane segments are connected by extracellular loops. While the precise function of CD133 remains unknown, it has been proposed that it acts as an organizer of cell membrane topology.

Background References

1. Kim MY et al. Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells. Oncol Lett 13:1767-1774 (2017).

2. Xi G et al. Targeting CD133 improves chemotherapeutic efficacy of recurrent pediatric pilocytic astrocytoma following prolonged chemotherapy. Mol Cancer 16:21 (2017).

Subcellular Location

Endoplasmic reticulum. Plasma membrane. Cell projection.

UNIPROT

SwissProt: O43490 Human

Synonyms

AC133 antibody

Antigen AC133 antibody

CD133 antibody

CORD12 antibody

Hematopoietic stem cell antigen antibody

hProminin antibody

MCDR2 antibody

MSTP061 antibody

OTTHUMP00000217744 antibody

OTTHUMP00000217745 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (<a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>) at 1/1,000 dilution. <br /> <br />Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate <br />Lane 2: 293T (Human embryonic kidney cell) cell lysate <br />Lane 3: HT-29 (Human colorectal adenocarcinoma cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 1 minute 34 seconds; ECL: K1802 <br /> <br />293T is a negative control (PMID: 30783186). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 97 kDa <br />Observed band size: 120 kDa

    ☑ Relative expression (RE)

    This data was developed using HA724636, the same antibody clone in a different buffer formulation.
    Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (HA724636) at 1/1,000 dilution.

    Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate
    Lane 2: 293T (Human embryonic kidney cell) cell lysate
    Lane 3: HT-29 (Human colorectal adenocarcinoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minute 34 seconds; ECL: K1802

    293T is a negative control (PMID: 30783186).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724636, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 97 kDa
    Observed band size: 120 kDa

  • This data was developed using <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724636, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724636, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724636, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724636, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a>, the same antibody clone in a different buffer formulation.<br />Application: Flow Cytometry <br /> <br />Species: Human <br />Sample: 293T (Human embryonic kidney cells) (Left, Negative control) / Caco-2 (Human colorectal adenocarcinoma cell) (Right) <br /> <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724636" style="font-weight: bold;text-decoration: underline;">HA724636</a> (1/500, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />Negative control: 293T (PMID: 30783186).

    ☑ Relative expression (RE)

    This data was developed using HA724636, the same antibody clone in a different buffer formulation.
    Application: Flow Cytometry

    Species: Human
    Sample: 293T (Human embryonic kidney cells) (Left, Negative control) / Caco-2 (Human colorectal adenocarcinoma cell) (Right)

    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724636 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

    Negative control: 293T (PMID: 30783186).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"