CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71]
Catalog# HA724636
CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71]
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WB
-
IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD133 Recombinant Rabbit Monoclonal Antibody [PSH26-71]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 97 kDa
Positive Control
Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH26-71
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IHC-P
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1:500-1:1,000
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FC
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1:1,000
Target
Function
CD133 antigen, also known as prominin-1, is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins, which specifically localize to cellular protrusions. When embedded in the cell membrane, the membrane topology of prominin-1 is such that the N-terminus extends into the extracellular space and the C-terminus resides in the intracellular compartment. The protein consists of five transmembrane segments, with the first and second segments and the third and fourth segments connected by intracellular loops while the second and third as well as fourth and fifth transmembrane segments are connected by extracellular loops. While the precise function of CD133 remains unknown, it has been proposed that it acts as an organizer of cell membrane topology.
Background References
1. Kim MY et al. Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells. Oncol Lett 13:1767-1774 (2017).
2. Xi G et al. Targeting CD133 improves chemotherapeutic efficacy of recurrent pediatric pilocytic astrocytoma following prolonged chemotherapy. Mol Cancer 16:21 (2017).
Subcellular Location
Endoplasmic reticulum. Plasma membrane. Cell projection.
UNIPROT
Synonyms
AC133 antibody
Antigen AC133 antibody
CD133 antibody
CORD12 antibody
Hematopoietic stem cell antigen antibody
hProminin antibody
MCDR2 antibody
MSTP061 antibody
OTTHUMP00000217744 antibody
OTTHUMP00000217745 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (HA724636) at 1/1,000 dilution.
Lane 1: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate
Lane 2: 293T (Human embryonic kidney cell) cell lysate
Lane 3: HT-29 (Human colorectal adenocarcinoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute 34 seconds; ECL: K1802
293T is a negative control (PMID: 30783186).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724636, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 97 kDa
Observed band size: 120 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724636, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724636, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Flow Cytometry
Species: Human
Sample: 293T (Human embryonic kidney cells) (Left, Negative control) / Caco-2 (Human colorectal adenocarcinoma cell) (Right)
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724636 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: 293T (PMID: 30783186).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"