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PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752057

PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC(Intra)

Target Molecular Weight

Predicted band size: 123 kDa

Positive Control

A-204 (Human rhabdomyosarcoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate.

Conjugation

unconjugated

Clone Number

PSH27-00

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:100

  • FC(Intra)

  • 1:1,000

Target

Function

Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and -β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-β gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.

Background References

1. Zhang W et al. Immunohistochemical analysis of PDGFR-alpha for wound age determination. Int J Legal Med. 2024 Jul

2. Wang J et al. PDGFR-alpha shRNA-polyplex for uveal melanoma treatment via EMT mediated vasculogenic mimicry interfering. J Nanobiotechnology. 2024 Dec

Subcellular Location

Cell membrane, Cell projection, cilium, Golgi apparatus.

UNIPROT

SwissProt: P16234 Human

SwissProt: P26618 Mouse

SwissProt: P20786 Rat

Synonyms

Alpha-type platelet-derived growth factor receptor antibody

CD140 antigen-like family member A antibody

CD140a antibody

CD140a antigen antibody

MGC74795 antibody

PDGF alpha chain antibody

PDGF-R-alpha antibody

PDGFR 2 antibody

PDGFR alpha antibody

PDGFR2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of PDGFR alpha on different lysates with Rabbit anti-PDGFR alpha antibody (<a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a>) at 1/5,000 dilution. <br /> <br />Lane 1: A-204 (Human rhabdomyosarcoma cell) cell lysate <br />Lane 2: A-172 (Human brain glioblastoma cell) cell lysate <br />Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 1 minute 2 seconds ; ECL: K1801 <br /> <br />A172 is a negative control (PMID: 8425771). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 123 kDa <br />Observed band size: 150-200 kDa

    ☑ Relative expression (RE)

    Western blot analysis of PDGFR alpha on different lysates with Rabbit anti-PDGFR alpha antibody (HA752057) at 1/5,000 dilution.

    Lane 1: A-204 (Human rhabdomyosarcoma cell) cell lysate
    Lane 2: A-172 (Human brain glioblastoma cell) cell lysate
    Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minute 2 seconds ; ECL: K1801

    A172 is a negative control (PMID: 8425771).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA752057, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 123 kDa
    Observed band size: 150-200 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: A-204 (Human rhabdomyosarcoma cell),/,A-172 (Human brain glioblastoma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). <br /> <br />Negative control: A-172 (PMID: 8425771).

    ☑ Relative expression (RE)

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: A-204 (Human rhabdomyosarcoma cell),/,A-172 (Human brain glioblastoma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA752057, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Negative control: A-172 (PMID: 8425771).

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Mouse <br />Sample: NIH/3T3 (Mouse fibroblast) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblast)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA752057, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Rat <br />Sample: C6 (Rat glioma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a>, 1/50, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Rat
    Sample: C6 (Rat glioma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA752057, 1/50, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Flow Cytometry (Intra) <br /> <br />Species: Human <br />Sample: A-172 (Human brain glioblastoma cell) (Left, Negative) / A-204 (Human rhabdomyosarcoma cell) (Right) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />Negative control: A-172 (PMID: 8425771).

    ☑ Relative expression (RE)

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: A-172 (Human brain glioblastoma cell) (Left, Negative) / A-204 (Human rhabdomyosarcoma cell) (Right)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA752057 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

    Negative control: A-172 (PMID: 8425771).

  • Application: Flow Cytometry (Intra) <br /> <br />Species: Mouse <br />Sample: NIH/3T3 (Mouse fibroblasts) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />

    Application: Flow Cytometry (Intra)

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblasts)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA752057 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

  • Application: Flow Cytometry (Intra) <br /> <br />Species: Rat <br />Sample: C6 (Rat glioma cells) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA752057" style="font-weight: bold;text-decoration: underline;">HA752057</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />

    Application: Flow Cytometry (Intra)

    Species: Rat
    Sample: C6 (Rat glioma cells)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA752057 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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