PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00]
Catalog# HA724661
PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00]
-
WB
-
IF-Cell
-
FC(Intra)
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC(Intra)
Target Molecular Weight
Predicted band size: 123 kDa
Positive Control
A-204 (Human rhabdomyosarcoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate.
Conjugation
unconjugated
Clone Number
PSH27-00
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IF-Cell
-
1:50-1:100
-
FC(Intra)
-
1:1,000
Target
Function
Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and -β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-β gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.
Background References
1. Zhang W et al. Immunohistochemical analysis of PDGFR-alpha for wound age determination. Int J Legal Med. 2024 Jul
2. Wang J et al. PDGFR-alpha shRNA-polyplex for uveal melanoma treatment via EMT mediated vasculogenic mimicry interfering. J Nanobiotechnology. 2024 Dec
Subcellular Location
Cell membrane, Cell projection, cilium, Golgi apparatus.
Synonyms
Alpha-type platelet-derived growth factor receptor antibody
CD140 antigen-like family member A antibody
CD140a antibody
CD140a antigen antibody
MGC74795 antibody
PDGF alpha chain antibody
PDGF-R-alpha antibody
PDGFR 2 antibody
PDGFR alpha antibody
PDGFR2 antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of PDGFR alpha on different lysates with Rabbit anti-PDGFR alpha antibody (HA724661) at 1/5,000 dilution.
Lane 1: A-204 (Human rhabdomyosarcoma cell) cell lysate
Lane 2: A-172 (Human brain glioblastoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute 2 seconds ; ECL: K1801
A172 is a negative control (PMID: 8425771).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724661, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 123 kDa
Observed band size: 150-200 kDa -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: A-204 (Human rhabdomyosarcoma cell),/,A-172 (Human brain glioblastoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724661, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Negative control: A-172 (PMID: 8425771). -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724661, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: C6 (Rat glioma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724661, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
☑ Relative expression (RE)
Application: Flow Cytometry (Intra)
Species: Human
Sample: A-172 (Human brain glioblastoma cell) (Left, Negative) / A-204 (Human rhabdomyosarcoma cell) (Right)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724661 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: A-172 (PMID: 8425771). -
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724661 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
-
Application: Flow Cytometry (Intra)
Species: Rat
Sample: C6 (Rat glioma cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724661 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH27-00] - BSA and Azide free
Application: WB,IF-Cell,FC(Intra)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
PDGFR alpha Recombinant Rabbit Monoclonal Antibody [JF104-6]
Application: WB,IHC-Fr
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
Human PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH26-33] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
Biotin Conjugated Human PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH26-34] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human
Conjugate: Biotin
