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c-Myb Recombinant Rabbit Monoclonal Antibody [PSH27-17] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752070

c-Myb Recombinant Rabbit Monoclonal Antibody [PSH27-17] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

c-Myb Recombinant Rabbit Monoclonal Antibody [PSH27-17] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human c-Myb aa 151-640.

Species Reactivity

Human

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 72 kDa

Positive Control

RPMI8226 (Human multiple myeloma cell) cell lysate, SW620 (Human colon cancer cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, MOLT-4 (Human acute T-Cell leukemia cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, U-937 (Human acute monocytic leukemia cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH27-17

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1-2μg/sample

Target

Function

Myb genes are part of a large gene family of transcription factors found in animals and plants. In humans, it includes Myb proto-oncogene like 1 and Myb-related protein B in addition to MYB proper. Members of the extended SANT/Myb family also include the SANT domain and other similar all-helical homeobox-like domains. The Myb gene family is named after the eponymous gene in Avian myeloblastosis virus. The viral Myb (v-Myb, P01104) recognizes the sequence 5'-YAACKG-3'. It causes myeloblastosis (myeloid leukemia) in chickens. Compared to the normal animal cellular Myb (c-myb), v-myb contains deletions in the C-terminal regulatory domain, leading to aberrant activation of other oncogenes.

Background References

1. Říhová K et al. Transcription factor c-Myb: novel prognostic factor in osteosarcoma. Clin Exp Metastasis. 2022 Apr

2. Bu C et al. c-Myb protects cochlear hair cells from cisplatin-induced damage via the PI3K/Akt signaling pathway. Cell Death Discov. 2022 Feb

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P10242 Human

Synonyms

C myb antibody

c myb protein (140 AA) antibody

c-myb protein (140 AA) antibody

c-myb_CDS antibody

c-myb10A_CDS antibody

c-myb13A_CDS antibody

c-myb14A_CDS antibody

c-myb8B_CDS antibody

Cmyb antibody

efg antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (<a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a>) at 1/5,000 dilution. <br /> <br />Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate <br />Lane 2: SW620 (Human colon cancer cell) cell lysate <br />Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1802 <br /> <br />Negative expression of c-Myb protein in A549 is consistent with the predicted expression pattern. <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 72 kDa <br />Observed band size: 72-80 kDa

    ☑ Relative expression (RE)

    Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA752070) at 1/5,000 dilution.

    Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate
    Lane 2: SW620 (Human colon cancer cell) cell lysate
    Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1802

    Negative expression of c-Myb protein in A549 is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA752070, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 72 kDa
    Observed band size: 72-80 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (<a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a>) at 1/5,000 dilution. <br /> <br />Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate <br />Lane 2: MOLT-4 (Human acute T-Cell leukemia cell) cell lysate <br />Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate <br />Lane 4: THP-1 (Human acute monoblastic leukemia cell) cell lysate <br />Lane 5: U-937 (Human acute monocytic leukemia cell) cell lysate <br />Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1801 <br /> <br />Negative expression of c-Myb protein in HeLa is consistent with the predicted expression pattern. <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 72 kDa <br />Observed band size: 72-80 kDa

    ☑ Relative expression (RE)

    Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA752070) at 1/5,000 dilution.

    Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 2: MOLT-4 (Human acute T-Cell leukemia cell) cell lysate
    Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate
    Lane 4: THP-1 (Human acute monoblastic leukemia cell) cell lysate
    Lane 5: U-937 (Human acute monocytic leukemia cell) cell lysate
    Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Negative expression of c-Myb protein in HeLa is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA752070, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 72 kDa
    Observed band size: 72-80 kDa

  • Immunoprecipitation (IP) <br /> <br />c-Myb was immunoprecipitated in 0.2 mg Jurkat (Human T-lymphoblastic cells) cell lysate with <a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a> at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature. <br /> <br />Lane 1: Jurkat cell lysate (input) <br />Lane 2: <a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a> IP in Jurkat cell lysate <br />Lane 3: Rabbit IgG instead of <a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a> in Jurkat cell lysate <br /> <br />Exposure time: 20 seconds <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Primary dilution: <a href="/products/HA752070" style="font-weight: bold;text-decoration: underline;">HA752070</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature <br /> <br />Predicted band size: 72 kDa <br />Observed band size: 72-80 kDa

    Immunoprecipitation (IP)

    c-Myb was immunoprecipitated in 0.2 mg Jurkat (Human T-lymphoblastic cells) cell lysate with HA752070 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA752070 at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Jurkat cell lysate (input)
    Lane 2: HA752070 IP in Jurkat cell lysate
    Lane 3: Rabbit IgG instead of HA752070 in Jurkat cell lysate

    Exposure time: 20 seconds
    Blocking: 5% NFDM/TBST, 1 hour at room temperature

    Primary dilution: HA752070, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 72 kDa
    Observed band size: 72-80 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Application: WB

Reactivity: Human

Conjugate: unconjugated