c-Myb Recombinant Rabbit Monoclonal Antibody [PSH27-17]
Catalog# HA724678
c-Myb Recombinant Rabbit Monoclonal Antibody [PSH27-17]
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WB
-
IP
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Human
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unconjugated
Overview
Product Name
c-Myb Recombinant Rabbit Monoclonal Antibody [PSH27-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human c-Myb aa 151-640.
Species Reactivity
Human
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 72 kDa
Positive Control
RPMI8226 (Human multiple myeloma cell) cell lysate, SW620 (Human colon cancer cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, MOLT-4 (Human acute T-Cell leukemia cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, U-937 (Human acute monocytic leukemia cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH27-17
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
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IP
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1-2μg/sample
Target
Function
Myb genes are part of a large gene family of transcription factors found in animals and plants. In humans, it includes Myb proto-oncogene like 1 and Myb-related protein B in addition to MYB proper. Members of the extended SANT/Myb family also include the SANT domain and other similar all-helical homeobox-like domains. The Myb gene family is named after the eponymous gene in Avian myeloblastosis virus. The viral Myb (v-Myb, P01104) recognizes the sequence 5'-YAACKG-3'. It causes myeloblastosis (myeloid leukemia) in chickens. Compared to the normal animal cellular Myb (c-myb), v-myb contains deletions in the C-terminal regulatory domain, leading to aberrant activation of other oncogenes.
Background References
1. Říhová K et al. Transcription factor c-Myb: novel prognostic factor in osteosarcoma. Clin Exp Metastasis. 2022 Apr
2. Bu C et al. c-Myb protects cochlear hair cells from cisplatin-induced damage via the PI3K/Akt signaling pathway. Cell Death Discov. 2022 Feb
Subcellular Location
Nucleus.
UNIPROT
Synonyms
C myb antibody
c myb protein (140 AA) antibody
c-myb protein (140 AA) antibody
c-myb_CDS antibody
c-myb10A_CDS antibody
c-myb13A_CDS antibody
c-myb14A_CDS antibody
c-myb8B_CDS antibody
Cmyb antibody
efg antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA724678) at 1/5,000 dilution.
Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate
Lane 2: SW620 (Human colon cancer cell) cell lysate
Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1802
Negative expression of c-Myb protein in A549 is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724678, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 72 kDa
Observed band size: 72-80 kDa -
☑ Relative expression (RE)
Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA724678) at 1/5,000 dilution.
Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 2: MOLT-4 (Human acute T-Cell leukemia cell) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 4: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 5: U-937 (Human acute monocytic leukemia cell) cell lysate
Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Negative expression of c-Myb protein in HeLa is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724678, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 72 kDa
Observed band size: 72-80 kDa -
Immunoprecipitation (IP)
c-Myb was immunoprecipitated in 0.2 mg Jurkat (Human T-lymphoblastic cells) cell lysate with HA724678 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724678 at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA724678 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA724678 in Jurkat cell lysate
Exposure time: 20 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724678, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 72 kDa
Observed band size: 72-80 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]
Application: WB
Reactivity: Human
Conjugate: unconjugated
