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CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-03] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752091

CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-03] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-03] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CYP2E1 aa 251-493.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 57 kDa

Positive Control

Mouse liver tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH28-03

Product Features

Form

Liquid

Concentration

0.5 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) .Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).

Background References

1. Gi M et al. Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-Dichloropropane in Male Syrian Hamsters and B6C3F1 Mice. Toxicol Sci 145:196-208 (2015).

2. Boussadia B et al. Effect of status epilepticus and antiepileptic drugs on CYP2E1 brain expression. Neuroscience 281C:124-134 (2014).

Subcellular Location

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.

UNIPROT

SwissProt: Q05421 Mouse

SwissProt: P05182 Rat

Synonyms

4-nitrophenol 2-hydroxylase antibody

CP2E1_HUMAN antibody

CPE1 antibody

CYP2E antibody

Cyp2e1 antibody

CYPIIE1 antibody

Cytochrome P450 2E1 antibody

Cytochrome P450 isozyme 3A antibody

Cytochrome P450 LM3A antibody

Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of CYP2E1 on different lysates with Rabbit anti-CYP2E1 antibody (<a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>) at 1/10,000 dilution. <br /> <br />Lane 1: Mouse liver tissue lysate <br />Lane 2: Mouse spleen tissue lysate <br />Lane 3: Rat liver tissue lysate <br />Lane 4: Rat spleen tissue lysate <br /> <br />Lysates/proteins at 10 µg/Lane. <br />Exposure time: 30 seconds; ECL: K1801 <br /> <br />Low expression: Spleen (PMID: 15576447). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 57 kDa <br />Observed band size: 50 kDa

    ☑ Relative expression (RE)

    This data was developed using HA724745, the same antibody clone in a different buffer formulation.
    Western blot analysis of CYP2E1 on different lysates with Rabbit anti-CYP2E1 antibody (HA724745) at 1/10,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Rat liver tissue lysate
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 30 seconds; ECL: K1801

    Low expression: Spleen (PMID: 15576447).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724745, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 57 kDa
    Observed band size: 50 kDa

  • This data was developed using <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724745, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724745, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    This data was developed using HA724745, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724745, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern.

  • This data was developed using <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724745, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724745, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    This data was developed using HA724745, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724745, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern.

  • This data was developed using <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724745" style="font-weight: bold;text-decoration: underline;">HA724745</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    This data was developed using HA724745, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724745, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

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