CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-03]
Catalog# HA724745
CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-03]
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WB
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IHC-P
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IF-Tissue
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Mouse
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Rat
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unconjugated
Overview
Product Name
CYP2E1 Recombinant Rabbit Monoclonal Antibody [PSH28-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse CYP2E1 aa 251-493.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
Mouse liver tissue lysate, Rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
PSH28-03
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | IF-Tissue | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:10,000
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IHC-P
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1:2,000-1:5,000
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IF-Tissue
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1:100
Target
Function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) .Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).
Background References
1. Gi M et al. Determination of Hepatotoxicity and Its Underlying Metabolic Basis of 1,2-Dichloropropane in Male Syrian Hamsters and B6C3F1 Mice. Toxicol Sci 145:196-208 (2015).
2. Boussadia B et al. Effect of status epilepticus and antiepileptic drugs on CYP2E1 brain expression. Neuroscience 281C:124-134 (2014).
Subcellular Location
Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.
Synonyms
4-nitrophenol 2-hydroxylase antibody
CP2E1_HUMAN antibody
CPE1 antibody
CYP2E antibody
Cyp2e1 antibody
CYPIIE1 antibody
Cytochrome P450 2E1 antibody
Cytochrome P450 isozyme 3A antibody
Cytochrome P450 LM3A antibody
Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CYP2E1 on different lysates with Rabbit anti-CYP2E1 antibody (HA724745) at 1/10,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Rat liver tissue lysate
Lane 4: Rat spleen tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Low expression: Spleen (PMID: 15576447).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724745, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 57 kDa
Observed band size: 50 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724745, 1/5,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724745, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724745, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724745, 1/5,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative expression of CYP2E1 protein in spleen is consistent with the predicted expression pattern. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724745, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"