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RUVBL2 Recombinant Antibody [A1E12-R] - Rabbit IgG (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752118

RUVBL2 Recombinant Antibody [A1E12-R] - Rabbit IgG (Chimeric) - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

RUVBL2 Recombinant Antibody [A1E12-R] - Rabbit IgG (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Human RUVB2 aa 130-321 / 463.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IF-Cell, IHC-P, FC(Intra)

Target Molecular Weight

Predicted band size: 51 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.

Conjugation

unconjugated

Clone Number

A1E12-R

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC(Intra) IF-Cell
human
Tested Tested
Tested Tested
Not Recommended
Not Recommended
mouse
Tested Tested
Tested Tested
Tested Tested
Tested Tested
rat
Tested Tested
Tested Tested
Not Recommended
Tested Tested
green monkey
Tested Tested
 
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.

Background References

1. Muñoz-Hernández H. et. al. Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM. Sci Adv. 2019 May 1;5(5):eaaw1616.

2. Morwitzer MJ. et. al. Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein. Viruses. 2019 Apr 23;11(4).

Subcellular Location

Nucleus matrix, nucleoplasm, cytoplasm, membrane.

UNIPROT

SwissProt: Q9Y230 Human

SwissProt: Q9WTM5 Mouse

Entrez Gene: 292907 Rat

Synonyms

48 kDa TATA box-binding protein-interacting protein antibody

48 kDa TBP-interacting protein antibody

48-kDa TATA box-binding protein-interacting protein antibody

48-kDa TBP-interacting protein antibody

51 kDa erythrocyte cytosolic protein antibody

CGI-46 antibody

EC=3.6.1.- antibody

ECP-51 antibody

ECP51 antibody

Erythrocyte cytosolic protein, 51-KD antibody

Expand

Images

  • This data was developed using <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of RUVBL2 on different lysates with Rabbit anti-RUVBL2 antibody (<a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>) at 1/2,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane) <br />Lane 2: 293T (Human embryonic kidney cell) cell lysate (15 µg/Lane) <br />Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate (15 µg/Lane) <br />Lane 4: C6 (Rat glioma cell) cell lysate (15 µg/Lane) <br />Lane 5: COS-1 (African green monkey kidney fibroblast) cell lysate (15 µg/Lane) <br />Lane 6: Mouse testis tissue lysate (30 µg/Lane) <br />Lane 7: Rat testis tissue lysate (30 µg/Lane) <br /> <br />Exposure time: 6 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 51 kDa <br />Observed band size: 51 kDa

    This data was developed using HA724802, the same antibody clone in a different buffer formulation.
    Western blot analysis of RUVBL2 on different lysates with Rabbit anti-RUVBL2 antibody (HA724802) at 1/2,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane)
    Lane 2: 293T (Human embryonic kidney cell) cell lysate (15 µg/Lane)
    Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate (15 µg/Lane)
    Lane 4: C6 (Rat glioma cell) cell lysate (15 µg/Lane)
    Lane 5: COS-1 (African green monkey kidney fibroblast) cell lysate (15 µg/Lane)
    Lane 6: Mouse testis tissue lysate (30 µg/Lane)
    Lane 7: Rat testis tissue lysate (30 µg/Lane)

    Exposure time: 6 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724802, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • This data was developed using <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Mouse <br />Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    This data was developed using HA724802, the same antibody clone in a different buffer formulation.
    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724802, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • This data was developed using <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Rat <br />Sample: C6 (Rat glioma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    This data was developed using HA724802, the same antibody clone in a different buffer formulation.
    Application: Immunocytochemistry (IF-cell)

    Species: Rat
    Sample: C6 (Rat glioma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724802, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • This data was developed using <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Testis <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724802, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Testis
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Testis <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724802, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Testis
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Testis <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724802, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Testis
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a>, the same antibody clone in a different buffer formulation.<br />Application: Flow Cytometry (Intra) <br /> <br />Species: Mouse <br />Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724802" style="font-weight: bold;text-decoration: underline;">HA724802</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />

    This data was developed using HA724802, the same antibody clone in a different buffer formulation.
    Application: Flow Cytometry (Intra)

    Species: Mouse
    Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724802 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

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