RUVBL2 Recombinant Antibody [A1E12-R] - Rabbit IgG (Chimeric)
Catalog# HA724802
RUVBL2 Recombinant Antibody [A1E12-R] - Rabbit IgG (Chimeric)
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WB
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IF-Cell
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IHC-P
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FC(Intra)
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
RUVBL2 Recombinant Antibody [A1E12-R] - Rabbit IgG (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Human RUVB2 aa 130-321 / 463.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell, IHC-P, FC(Intra)
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, 293T (Human embryonic kidney cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
A1E12-R
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| human |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:500
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IHC-P
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1:2,000
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FC(Intra)
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1:1,000
Target
Function
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.
Background References
1. Muñoz-Hernández H. et. al. Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM. Sci Adv. 2019 May 1;5(5):eaaw1616.
2. Morwitzer MJ. et. al. Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein. Viruses. 2019 Apr 23;11(4).
Subcellular Location
Nucleus matrix, nucleoplasm, cytoplasm, membrane.
Synonyms
48 kDa TATA box-binding protein-interacting protein antibody
48 kDa TBP-interacting protein antibody
48-kDa TATA box-binding protein-interacting protein antibody
48-kDa TBP-interacting protein antibody
51 kDa erythrocyte cytosolic protein antibody
CGI-46 antibody
EC=3.6.1.- antibody
ECP-51 antibody
ECP51 antibody
Erythrocyte cytosolic protein, 51-KD antibody
ExpandImages
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Western blot analysis of RUVBL2 on different lysates with Rabbit anti-RUVBL2 antibody (HA724802) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane)
Lane 2: 293T (Human embryonic kidney cell) cell lysate (15 µg/Lane)
Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate (15 µg/Lane)
Lane 4: C6 (Rat glioma cell) cell lysate (15 µg/Lane)
Lane 5: COS-1 (African green monkey kidney fibroblast) cell lysate (15 µg/Lane)
Lane 6: Mouse testis tissue lysate (30 µg/Lane)
Lane 7: Rat testis tissue lysate (30 µg/Lane)
Exposure time: 6 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724802, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724802, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: C6 (Rat glioma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724802, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Testis
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724802, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724802 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"