HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52] - BSA and Azide free
Catalog# HA751227
HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human HMGCS1 aa 421-520.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue, IP
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
HepG2 cell lysate, MCF7 cell lysate, HT-29 cell lysate, A549 cell lysate, U-2 OS cell lysate, human liver tissue, mouse liver tissue, rat liver tissue, human lung cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
PSH08-52
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
In biochemistry, hydroxymethylglutaryl-CoA synthase or HMG-CoA synthase EC 2.3.3.10 is an enzyme which catalyzes the reaction in which acetyl-CoA condenses with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). This reaction comprises the second step in the mevalonate-dependent isoprenoid biosynthesis pathway. HMG-CoA is an intermediate in both cholesterol synthesis and ketogenesis. This reaction is overactivated in patients with diabetes mellitus type 1 if left untreated, due to prolonged insulin deficiency and the exhaustion of substrates for gluconeogenesis and the TCA cycle, notably oxaloacetate. This results in shunting of excess acetyl-CoA into the ketone synthesis pathway via HMG-CoA, leading to the development of diabetic ketoacidosis. In humans, the protein is encoded by the HMGCS1 gene on chromosome 5.
Background References
1. Li K et al. CSN6-SPOP-HMGCS1 Axis Promotes Hepatocellular Carcinoma Progression via YAP1 Activation. Adv Sci (Weinh). 2024 Apr
2. Zhang X et al. Compartmentalized activities of HMGCS1 control cervical cancer radiosensitivity. Cell Signal. 2023 Jan
Subcellular Location
Cytoplasm.
Synonyms
3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody
3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
cytoplasmic antibody
EC 2.3.3.10 antibody
HMCS1_HUMAN antibody
HMG CoA synthase antibody
HMG-CoA synthase antibody
HMGCS antibody
HMGCS1 antibody
Hydroxymethylglutaryl CoA synthase, cytoplasmic antibody
ExpandImages
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This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA722998) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: U-2 OS cell lysate (20 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 55 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722998) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722998, the same antibody clone in a different buffer formulation.
HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with HA722998 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722998 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HT-29 cell lysate (input)
Lane 2: HA722998 IP in HT-29 cell lysate
Lane 3: Rabbit IgG instead of HA722998 in HT-29 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 37 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52]
Application: WB,IHC-P,IF-Tissue,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51]
Application: WB,IP
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
