Skip to content

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA751226

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human HMGCS1 aa 421-520.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 57 kDa

Positive Control

HepG2 cell lysate, MCF7 cell lysate, HT-29 cell lysate, A549 cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH08-51

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

In biochemistry, hydroxymethylglutaryl-CoA synthase or HMG-CoA synthase EC 2.3.3.10 is an enzyme which catalyzes the reaction in which acetyl-CoA condenses with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). This reaction comprises the second step in the mevalonate-dependent isoprenoid biosynthesis pathway. HMG-CoA is an intermediate in both cholesterol synthesis and ketogenesis. This reaction is overactivated in patients with diabetes mellitus type 1 if left untreated, due to prolonged insulin deficiency and the exhaustion of substrates for gluconeogenesis and the TCA cycle, notably oxaloacetate. This results in shunting of excess acetyl-CoA into the ketone synthesis pathway via HMG-CoA, leading to the development of diabetic ketoacidosis. In humans, the protein is encoded by the HMGCS1 gene on chromosome 5.

Background References

1. Li K et al. CSN6-SPOP-HMGCS1 Axis Promotes Hepatocellular Carcinoma Progression via YAP1 Activation. Adv Sci (Weinh). 2024 Apr

2. Zhang X et al. Compartmentalized activities of HMGCS1 control cervical cancer radiosensitivity. Cell Signal. 2023 Jan

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q01581 Human

SwissProt: Q8JZK9 Mouse

SwissProt: P17425 Rat

Synonyms

3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody

3-hydroxy-3-methylglutaryl coenzyme A synthase antibody

cytoplasmic antibody

EC 2.3.3.10 antibody

HMCS1_HUMAN antibody

HMG CoA synthase antibody

HMG-CoA synthase antibody

HMGCS antibody

HMGCS1 antibody

Hydroxymethylglutaryl CoA synthase, cytoplasmic antibody

Expand

Images

  • Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (<a href="/products/HA751226" style="font-weight: bold;text-decoration: underline;">HA751226</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: HT-29 cell lysate<br />Lane 4: A549 cell lysate<br />Lane 5: COS-1 cell lysate<br />Lane 6: C2C12 cell lysate<br />Lane 7: NIH/3T3 cell lysate<br />Lane 8: C6 cell lysate<br />Lane 9: PC-12 cell lysate<br />Lane 10: Rat liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751226" style="font-weight: bold;text-decoration: underline;">HA751226</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA751226) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: A549 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: C2C12 cell lysate
    Lane 7: NIH/3T3 cell lysate
    Lane 8: C6 cell lysate
    Lane 9: PC-12 cell lysate
    Lane 10: Rat liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 55 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751226) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with <a href="/products/HA751226" style="font-weight: bold;text-decoration: underline;">HA751226</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751226" style="font-weight: bold;text-decoration: underline;">HA751226</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HT-29 cell lysate (input)<br />Lane 2: <a href="/products/HA751226" style="font-weight: bold;text-decoration: underline;">HA751226</a> IP in HT-29 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751226" style="font-weight: bold;text-decoration: underline;">HA751226</a> in HT-29 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 37 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with HA751226 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751226 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HT-29 cell lysate (input)
    Lane 2: HA751226 IP in HT-29 cell lysate
    Lane 3: Rabbit IgG instead of HA751226 in HT-29 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 37 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51]

Application: WB,IP

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated

metafield image

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52]

Application: WB,IHC-P,IF-Tissue,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52] - BSA and Azide free

Application: WB,IHC-P,IF-Tissue,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated