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HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51]

Usd: 385

Specification

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Catalog# HA722997

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-51]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human HMGCS1 aa 421-520.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Target Molecular Weight

Predicted band size: 57 kDa

Positive Control

HepG2 cell lysate, MCF7 cell lysate, HT-29 cell lysate, A549 cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH08-51

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell IP
human
Tested Tested
Not Recommended
Not Recommended
Not Recommended
Tested Tested
mouse
Tested Tested
Not Recommended
Not Recommended
Not Recommended
 
rat
Tested Tested
Not Recommended
Not Recommended
Not Recommended
 
green monkey
Tested Tested
 
 
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

In biochemistry, hydroxymethylglutaryl-CoA synthase or HMG-CoA synthase EC 2.3.3.10 is an enzyme which catalyzes the reaction in which acetyl-CoA condenses with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). This reaction comprises the second step in the mevalonate-dependent isoprenoid biosynthesis pathway. HMG-CoA is an intermediate in both cholesterol synthesis and ketogenesis. This reaction is overactivated in patients with diabetes mellitus type 1 if left untreated, due to prolonged insulin deficiency and the exhaustion of substrates for gluconeogenesis and the TCA cycle, notably oxaloacetate. This results in shunting of excess acetyl-CoA into the ketone synthesis pathway via HMG-CoA, leading to the development of diabetic ketoacidosis. In humans, the protein is encoded by the HMGCS1 gene on chromosome 5.

Background References

1. Li K et al. CSN6-SPOP-HMGCS1 Axis Promotes Hepatocellular Carcinoma Progression via YAP1 Activation. Adv Sci (Weinh). 2024 Apr

2. Zhang X et al. Compartmentalized activities of HMGCS1 control cervical cancer radiosensitivity. Cell Signal. 2023 Jan

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q01581 Human

SwissProt: Q8JZK9 Mouse

SwissProt: P17425 Rat

Synonyms

3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody

3-hydroxy-3-methylglutaryl coenzyme A synthase antibody

cytoplasmic antibody

EC 2.3.3.10 antibody

HMCS1_HUMAN antibody

HMG CoA synthase antibody

HMG-CoA synthase antibody

HMGCS antibody

HMGCS1 antibody

Hydroxymethylglutaryl CoA synthase, cytoplasmic antibody

Expand

Images

  • Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (<a href="/products/HA722997" style="font-weight: bold;text-decoration: underline;">HA722997</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: HT-29 cell lysate<br />Lane 4: A549 cell lysate<br />Lane 5: COS-1 cell lysate<br />Lane 6: C2C12 cell lysate<br />Lane 7: NIH/3T3 cell lysate<br />Lane 8: C6 cell lysate<br />Lane 9: PC-12 cell lysate<br />Lane 10: Rat liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722997" style="font-weight: bold;text-decoration: underline;">HA722997</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA722997) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: A549 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: C2C12 cell lysate
    Lane 7: NIH/3T3 cell lysate
    Lane 8: C6 cell lysate
    Lane 9: PC-12 cell lysate
    Lane 10: Rat liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 55 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722997) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with <a href="/products/HA722997" style="font-weight: bold;text-decoration: underline;">HA722997</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722997" style="font-weight: bold;text-decoration: underline;">HA722997</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HT-29 cell lysate (input)<br />Lane 2: <a href="/products/HA722997" style="font-weight: bold;text-decoration: underline;">HA722997</a> IP in HT-29 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722997" style="font-weight: bold;text-decoration: underline;">HA722997</a> in HT-29 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 37 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with HA722997 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722997 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HT-29 cell lysate (input)
    Lane 2: HA722997 IP in HT-29 cell lysate
    Lane 3: Rabbit IgG instead of HA722997 in HT-29 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 37 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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HMGCS1 Recombinant Rabbit Monoclonal Antibody [PSH08-52] - BSA and Azide free

Application: WB,IHC-P,IF-Tissue,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated