Human CD163 Recombinant Rabbit Monoclonal Antibody [PSH13-80] - BSA and Azide free (Capture)

Sandwich ELISA analysis of human CD163 matched pair antibodies

Capture: HA723569, Human CD163 Rabbit mAb [PSH13-80]
Detector: HA723567, Human CD163 Rabbit mAb [PSH13-79]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723569) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD163 protein (HA211143) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723567, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD163 in human serum samples.

Capture: HA723569, Human CD163 Rabbit mAb [PSH13-80]
Detector: HA723567, Human CD163 Rabbit mAb [PSH13-79]

The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curve and corrected for sample dilution. Undiluted samples are human serum 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 745.9 ng/ml in human serum.

Interpolated concentrations of spiked CD163 in human cell culture media samples.

Capture: HA723569, Human CD163 Rabbit mAb [PSH13-80]
Detector: HA723567, Human CD163 Rabbit mAb [PSH13-79]

The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).