Biotin Conjugated Human CD85d Recombinant Rabbit Monoclonal Antibody [PSH05-62]
Overview
Product Name
Biotin Conjugated Human CD85d Recombinant Rabbit Monoclonal Antibody [PSH05-62]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CD85d aa 22-460 (Extracellular).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant standard Human CD85d protein (HA210643).
Conjugation
Biotin
Clone Number
PSH05-62
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-61] to Human CD85d (Capture) (HA722381) and recombinant standard Human CD85d protein (HA210643). The reference range value is 61.7-15,000 pg/mL.
Target
Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Background References
1. Koestlin N., Ostermeir A.L., Spring B., Schwarz J., Marme A., Walter C.B., Poets C.F., Gille C. HLA-G promotes myeloid-derived suppressor cell accumulation and suppressive activity during human pregnancy through engagement of the receptor ILT4. Eur. J. Immunol. 47:374-384 (2017).
2. Gregori S., Tomasoni D., Pacciani V., Scirpoli M., Battaglia M., Magnani C.F., Hauben E., Roncarolo M.G.. Differentiation of type 1 T regulatory cells (Tr1) by tolerogenic DC-10 requires the IL-10-dependent ILT4/HLA-G pathway. Blood 116:935-944 (2010).
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD85 antigen-like family member D antibody
CD85d antibody
CD85d antigen antibody
ILT 4 antibody
ILT-4 antibody
ILT4 antibody
Immunoglobulin like transcript 4 antibody
Immunoglobulin-like transcript 4 antibody
Leukocyte immunoglobulin like receptor 2 antibody
Leukocyte immunoglobulin like receptor subfamily B member 2 antibody
ExpandImages
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human CD85d Recombinant Rabbit Monoclonal Antibody [PSH05-62] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
