HRP Conjugated Human GDF15 Recombinant Rabbit Monoclonal Antibody [PSH16-02] - Detector
Overview
Product Name
HRP Conjugated Human GDF15 Recombinant Rabbit Monoclonal Antibody [PSH16-02] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human GDF15 aa 195-308 (HA211526).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human GDF-15 protein (HA211526).
Conjugation
HRP
Clone Number
PSH16-02
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| Elisa | |
|---|---|
| human |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-00] to Human GDF-15 antibody (Capture) (HA723805) and Recombinant Human GDF-15 protein (HA211526) as the standard. The reference range value is 9.77-1,250 pg/mL
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Growth/differentiation factor 15 is a protein that in humans is encoded by the GDF15 gene. GDF15 was first identified as Macrophage inhibitory cytokine-1 or MIC-1. It is a protein belonging to the transforming growth factor beta superfamily. Under normal conditions, GDF15 is expressed in low concentrations in most organs and upregulated because of injury of organs such as liver, kidney, heart and lung. The function of GDF15 is not fully clear but it seems to have a role in regulating inflammatory pathways and to be involved in regulating apoptosis, angiogenesis, cell repair and cell growth, which are biological processes observed in cardiovascular and neoplastic disorders.
Background References
1. Wang D et al. GDF15: emerging biology and therapeutic applications for obesity and cardiometabolic disease. Nat Rev Endocrinol. 2021 Oct
2. Conte M et al. GDF15, an emerging key player in human aging. Ageing Res Rev. 2022 Mar
Subcellular Location
Secreted.
UNIPROT
Synonyms
GDF 15 antibody
GDF-15 antibody
Gdf15 antibody
GDF15_HUMAN antibody
Growth differentiation factor 15 antibody
Growth/differentiation factor 15 antibody
Macrophage inhibitory cytokine 1 antibody
MIC 1 antibody
Mic-1 antibody
MIC1 antibody
ExpandImages
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![Sandwich ELISA analysis of Human GDF15 matched pair antibodies<br /><br />Capture: <a href="/products/HA723805" style="font-weight: bold;text-decoration: underline;">HA723805</a>, Human GDF-15 Rabbit mAb [PSH16-00]<br />Detector: <a href="/products/HA723807" style="font-weight: bold;text-decoration: underline;">HA723807</a>, Human GDF-15 Rabbit mAb [PSH16-02]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723805" style="font-weight: bold;text-decoration: underline;">HA723805</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein (<a href="/products/HA211526" style="font-weight: bold;text-decoration: underline;">HA211526</a>) starting from 1,250 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723807" style="font-weight: bold;text-decoration: underline;">HA723807</a>, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723807H_1.jpg?v=20260915230409)
Sandwich ELISA analysis of Human GDF15 matched pair antibodies
Capture: HA723805, Human GDF-15 Rabbit mAb [PSH16-00]
Detector: HA723807, Human GDF-15 Rabbit mAb [PSH16-02]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723805) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human GDF-15 protein (HA211526) starting from 1,250 pg/ml to 0 pg/ml and detect antibody (HA723807, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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