Human IL-2 Receptor alpha Recombinant Rabbit Monoclonal Antibody [PS01-03] - BSA and Azide free (Detector)
Overview
Product Name
Human IL-2 Receptor alpha Recombinant Rabbit Monoclonal Antibody [PS01-03] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within full length human IL2 Receptor alpha.
Species Reactivity
Human
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 31 kDa
Positive Control
Recombinant IL-2 Receptor alpha protein
Conjugation
unconjugated
Clone Number
PS01-03
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA (Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS00-52] to IL-2 Receptor alpha (Capture) (HA721280).
Target
Function
Interleukin-2 receptor alpha chain (also called CD25) is the human protein encoded by the IL2RA gene. The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolysis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is currently unknown. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. IL2RA is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, mastocytosis and tumor infiltrating lymphocytes. It functions as the receptor for HTLV-1 and is consequently expressed on neoplastic cells in adult T cell lymphoma/leukemia. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.
Background References
1. Triplett, Todd A.; et al. (July 2012). "Defining a functionally distinct subset of human memory CD4+ T cells that are CD25POS and FOXP3NEG". European Journal of Immunology. 42 (7): 1893–905.
2. Bezrodnik L., Caldirola M.S., Seminario A.G., Moreira I., Gaillard M.I. Follicular bronchiolitis as phenotype associated with CD25 deficiency. Clin. Exp. Immunol. 175:227-234 (2014)
Subcellular Location
Membrane.
UNIPROT
Synonyms
Interleukin 2 receptor alpha chain antibody
CD25 antibody
CD25 antigen antibody
IDDM10 antibody
IL 2 receptor alpha subunit antibody
IL-2 receptor subunit alpha antibody
IL-2-RA antibody
IL-2R subunit alpha antibody
IL2 RA antibody
IL2 Receptor alpha antibody
ExpandImages
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The binding activity of IL-2 Receptor alpha (HA721281) with recombinant IL-2 Receptor alpha protein.
Immobilized recombinant IL-2 Receptor alpha protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721281) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Standard curve of IL-2 Receptor alpha matched pair antibodies:<br /><br />Sandwich ELISA analysis of IL-2 Receptor alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody <a href="/products/HA721280" style="font-weight: bold;text-decoration: underline;">HA721280</a> [PS00-52] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IL-2 Receptor alpha protein starting from 2000 pg/ml to 31.25 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS01-03] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721281_2.jpg?v=20260429161637)
Standard curve of IL-2 Receptor alpha matched pair antibodies:
Sandwich ELISA analysis of IL-2 Receptor alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721280 [PS00-52] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IL-2 Receptor alpha protein starting from 2000 pg/ml to 31.25 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS01-03] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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