Human IL-7 Recombinant Rabbit Monoclonal Antibody [PSH03-10] - BSA and Azide free (Detector)
Overview
Product Name
Human IL-7 Recombinant Rabbit Monoclonal Antibody [PSH03-10] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IL-7 aa 26-177.
Species Reactivity
Human
Validated Applications
ELISA(Det)
Target Molecular Weight
Predicted band size: 20.2 kDa
Positive Control
Recombinant Human Human IL-7 (HA210595).
Conjugation
unconjugated
Clone Number
PSH03-10
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-09] to Human Human IL-7 (Capture) (HA721937) and recombinant standard Human Human IL-7 (HA210595) as the standard. The reference range value is 8.2-2,000 pg/mL.
Target
Function
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
Background References
1. Bazdar D.A., Kalinowska M., Panigrahi S., Sieg S.F. Recycled IL-7 Can Be Delivered to Neighboring T Cells. J. Immunol. 194:4698-4704 (2015).
2. Kondo M., Takeshita T., Higuchi M., Nakamura M., Sudo T., Nishikawa S., Sugamura K. Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes. Science 263:1453-1454 (1994).
Subcellular Location
Secreted.
UNIPROT
Synonyms
IL 7 antibody
IL-7 antibody
Il7 antibody
IL7_HUMAN antibody
Interleukin 7 antibody
Interleukin-7 antibody
LP-1 antibody
Lymphopoietin 1 antibody
Images
-
Sandwich ELISA analysis of human IL-7 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721937) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-7 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721938) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Biotin Conjugated Human IL-7 Recombinant Rabbit Monoclonal Antibody [PSH03-10] - Detector
Application: ELISA(Det)
Reactivity: Human
Conjugate: Biotin
IL-7 Mouse Monoclonal Antibody [A8B11]
Application: WB
Reactivity: Human
Conjugate: unconjugated
Human IL-7 Recombinant Rabbit Monoclonal Antibody [PSH03-09] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
