Biotin Conjugated Human/Monkey Pro-COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH25-16] - Detector
Catalog# HA724510B
Biotin Conjugated Human/Monkey Pro-COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH25-16] - Detector
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ELISA(Det)
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ELISA
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Human
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Rhesus monkey
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19.5-2,500 pg/mL
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6.75
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Biotin
Overview
Product Name
Biotin Conjugated Human/Monkey Pro-COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH25-16] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Pro-COL1A1 aa 23-161 (HA211375).
Species Reactivity
Human, Rhesus monkey
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Pro-COL1A1 protein (HA211375).
Conjugation
Biotin
Clone Number
PSH25-16
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH25-15] to Human/Monkey Pro-COL1A1 (Capture) (HA724509) and Recombinant Human Pro-COL1A1 protein (HA211375) as the standard. The reference range value is 19.5-2,500 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Collagen, type I, alpha 1, also known as alpha-1 type I collagen, is a protein that in humans is encoded by the COL1A1 gene. COL1A1 encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. Collagen is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera). The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain. This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong mature type I collagen fibers. Collagenous function includes rigidity and elasticity.
Background References
1. Liu Q et al. COL1A1-positive endothelial cells promote gastric cancer progression via the ANGPTL4-SDC4 axis driven by endothelial-to-mesenchymal transition. Cancer Lett. 2025 Jul
2. Saito M et al. Is COL1A1 Gene rs1107946 Polymorphism Associated with Sport Climbing Status and Flexibility? Genes (Basel). 2022 Feb
Subcellular Location
Secreted, extracellular space, extracellular matrix.
UNIPROT
Synonyms
Alpha 1 type I collagen antibody
alpha1(I) procollagen antibody
CO1A1_HUMAN antibody
COL1A1 antibody
collagen alpha 1 chain type I antibody
Collagen alpha-1(I) chain antibody
collagen alpha-1(I) chain preproprotein antibody
Collagen I alpha 1 polypeptide antibody
collagen of skin, tendon and bone, alpha-1 chain antibody
Collagen type I alpha 1 antibody
ExpandImages
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Sandwich ELISA analysis of Human Pro-COL1A1 matched pair antibodies
Capture: HA724509, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-15]
Detector: HA724510, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-16]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724509) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Pro-COL1A1 protein (HA211375) starting from 2,500 pg/mL to 0 pg/mL and detect antibody (HA724510, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native Pro-COL1A1 in human and monkey serum.
Capture: HA724509, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-15]
Detector: HA724510, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-16]
Interpolated concentration of native Pro-COL1A1 was measured in duplicate at different sample concentrations and interpolated from the Pro-COL1A1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-COL1A1 concentration was determined to be 122,731 pg/mL in rhesus monkey serum and 25,357 pg/mL in human serum. -
Interpolated concentrations of spiked Pro-COL1A1 in cell culture media samples.
Capture: HA724509, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-15]
Detector: HA724510, Human/Monkey Pro-COL1A1 Rabbit mAb [PSH25-16]
The concentrations of Pro-COL1A1 were measured in duplicates, interpolated from the Pro-COL1A1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human/Monkey Pro-COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH25-15] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Rhesus monkey
Conjugate: unconjugated
