Biotin Conjugated Human TREM1 Recombinant Rabbit Monoclonal Antibody [PSH17-86] - Detector
Overview
Product Name
Biotin Conjugated Human TREM1 Recombinant Rabbit Monoclonal Antibody [PSH17-86] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TREM1 aa 21-205 (HA210846).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human TREM1 protein (HA210846).
Conjugation
Biotin
Clone Number
PSH17-86
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-85] to Human TREM1 antibody (Capture) (HA725233) and Recombinant Human TREM1 protein (HA210846) as the standard. The reference range value is 15.6-8,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Triggering receptor expressed on myeloid cells 1 (TREM1) is an immunoglobulin (Ig) superfamily transmembrane protein that, in humans, is encoded by the TREM1 gene. TREM1 is constitutively expressed on the surface of peripheral blood monocytes and neutrophils, and upregulated by toll-like receptor (TLR) ligands; activation of TREM1 amplifies immune responses. Monocyte-, macrophage- and neutrophil-mediated inflammatory responses can be stimulated via receptors such as G protein-linked 7-transmembrane receptors (such as FPR1), Fc receptors, CD14, TLRs (such as TLR4), and cytokine receptors. TREM1 amplifies TLR-induced inflammation by increasing production of inflammatory cytokines. The ligand of TREM1 is unknown, however, bacterial infection, ischemic stroke and challenge with lipopolysaccharide or lipoteichoic acid were observed to increase TREM1 expression. In granulocytes, C/EBPε induces TREM1 expression independently from inflammatory response. TREM1 forms a complex with transmembrane adaptor DAP12 and, upon TREM1 engagement, a protein tyrosine kinase Syk-mediated cascade of tyrosine phosphorylation is initiated, activating downstream mediators such as PLCγ, PI3K, and MAPK. This cascade promotes the release of inflammatory cytokines and/or chemokines by neutrophils and macrophages, as well as their migration.
Background References
1. Li Q et al. Impaired lipophagy induced-microglial lipid droplets accumulation contributes to the buildup of TREM1 in diabetes-associated cognitive impairment. Autophagy. 2023 Oct
2. Li C et al. TREM1: Activation, signaling, cancer and therapy. Pharmacol Res. 2024 Jun
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
CD354 antibody
OTTMUSP00000018206 antibody
TREM 1 antibody
TREM-1 antibody
TREM1 antibody
TREM1_HUMAN antibody
Triggering receptor expressed on monocytes 1 antibody
Triggering receptor expressed on myeloid cells 1 antibody
Triggering receptor TREM 1 antibody
Triggering receptor TREM1 antibody
Images
-
![Sandwich ELISA analysis of Human TREM1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725233" style="font-weight: bold;text-decoration: underline;">HA725233</a>, Human TREM1 Rabbit mAb [PSH17-85]<br />Detector: <a href="/products/HA725234" style="font-weight: bold;text-decoration: underline;">HA725234</a>, Human TREM1 Rabbit mAb [PSH17-86]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725233" style="font-weight: bold;text-decoration: underline;">HA725233</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TREM1 protein (<a href="/products/HA210846" style="font-weight: bold;text-decoration: underline;">HA210846</a>) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725234" style="font-weight: bold;text-decoration: underline;">HA725234</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725234B_1.png?v=20260604174726)
Sandwich ELISA analysis of Human TREM1 matched pair antibodies
Capture: HA725233, Human TREM1 Rabbit mAb [PSH17-85]
Detector: HA725234, Human TREM1 Rabbit mAb [PSH17-86]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725233) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TREM1 protein (HA210846) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725234, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native TREM1 in mouse kidney samples.<br /><br />Capture: <a href="/products/HA725233" style="font-weight: bold;text-decoration: underline;">HA725233</a>, Human TREM1 Rabbit mAb [PSH17-85]<br />Detector: <a href="/products/HA725234" style="font-weight: bold;text-decoration: underline;">HA725234</a>, Human TREM1 Rabbit mAb [PSH17-86]<br /><br />The concentrations of TREM1 were measured in duplicates, interpolated from the TREM1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM1 concentration was determined to be 195,813 pg/ml in mouse kidney.](https://storage.huabio.cn/huabio/productImg/HA725234B_2.png?v=20260604174726)
Interpolated concentrations of native TREM1 in mouse kidney samples.
Capture: HA725233, Human TREM1 Rabbit mAb [PSH17-85]
Detector: HA725234, Human TREM1 Rabbit mAb [PSH17-86]
The concentrations of TREM1 were measured in duplicates, interpolated from the TREM1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM1 concentration was determined to be 195,813 pg/ml in mouse kidney.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
TREM1 Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Tissue,FC
Reactivity: Human,Rat
Conjugate: unconjugated
Human TREM1 Recombinant Rabbit Monoclonal Antibody [PSH17-85] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
