HRP Conjugated Human c-Kit Recombinant Rabbit Monoclonal Antibody [PSH26-41] - Detector
Overview
Product Name
HRP Conjugated Human c-Kit Recombinant Rabbit Monoclonal Antibody [PSH26-41] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human c-Kit aa 26-516 (HA211233).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human c-Kit protein (HA211233).
Conjugation
HRP
Clone Number
PSH26-41
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-40] to Human c-Kit (Capture) (HA724619) and Recombinant Human c-Kit protein (HA211233) as the standard. The reference range value is 23.44-3,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Proto-oncogene c-KIT is the gene encoding the receptor tyrosine kinase protein known as tyrosine-protein kinase KIT, CD117 (cluster of differentiation 117) or mast/stem cell growth factor receptor (SCFR). Multiple transcript variants encoding different isoforms have been found for this gene. KIT was first described by the German biochemist Axel Ullrich in 1987 as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. Altered forms of this receptor may be associated with some types of cancer. KIT is a receptor tyrosine kinase type III, which binds to stem cell factor, also known as "steel factor" or "c-kit ligand". When this receptor binds to stem cell factor (SCF) it forms a dimer that activates its intrinsic tyrosine kinase activity, that in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. After activation, the receptor is ubiquitinated to mark it for transport to a lysosome and eventual destruction. Signaling through KIT plays a role in cell survival, proliferation, and differentiation. For instance, KIT signaling is required for melanocyte survival, and it is also involved in haematopoiesis and gametogenesis.
Background References
1. Harris KS. et. al. CD117/c-kit defines a prostate CSC-like subpopulation driving progression and TKI resistance. Sci Rep. 2021 Jan
2. Russkamp NF. et. al. Anti-CD117 immunotherapy to eliminate hematopoietic and leukemia stem cells. Exp Hematol. 2021 Mar
Subcellular Location
Cell membrane; Cytoplasm.
UNIPROT
Synonyms
C Kit antibody
c-Kit antibody
c-Kit Ligand antibody
CD117 antibody
Kit antibody
Kit Ligand antibody
KIT oncogene antibody
KIT proto oncogene receptor tyrosine kinase antibody
KIT_HUMAN antibody
Mast cell growth factor receptor antibody
ExpandImages
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![Sandwich ELISA analysis of Human c-Kit matched pair antibodies<br /><br />Capture: <a href="/products/HA724619" style="font-weight: bold;text-decoration: underline;">HA724619</a>, Human c-Kit Rabbit mAb [PSH26-40]<br />Detector: <a href="/products/HA724620" style="font-weight: bold;text-decoration: underline;">HA724620</a>, Human c-Kit Rabbit mAb [PSH26-41]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA724619" style="font-weight: bold;text-decoration: underline;">HA724619</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human c-Kit protein (<a href="/products/HA211233" style="font-weight: bold;text-decoration: underline;">HA211233</a>) starting from 3,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA724620" style="font-weight: bold;text-decoration: underline;">HA724620</a>, HRP, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA724620H_1.jpg?v=20260920095215)
Sandwich ELISA analysis of Human c-Kit matched pair antibodies
Capture: HA724619, Human c-Kit Rabbit mAb [PSH26-40]
Detector: HA724620, Human c-Kit Rabbit mAb [PSH26-41]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724619) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human c-Kit protein (HA211233) starting from 3,000 pg/mL to 0 pg/mL and detect antibody (HA724620, HRP, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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