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c-Kit Recombinant Rabbit Monoclonal Antibody

Usd: 385

Specification

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Catalog# IRS013

c-Kit Recombinant Rabbit Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

c-Kit Recombinant Rabbit Monoclonal Antibody

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human c-Kit aa 950-976.

Species Reactivity

Human

Validated Applications

mIHC

Molecular Weight

Predicted band size: 110 kDa

Positive Control

Human tonsils tissue, human lung cancer tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100

Target

Function

CD117 is a 145-160 kDa cell membrane protein encoded by the c-kit proto-oncogene. CD117 is required for the development and growth of a large number of cells expressing this protein. The cells (particularly the mast cells) show a strong membrane as well as cytoplasmic staining. CD117 moreover is expressed in various epithelia (breast, sweat glands and salivary glands, renal tubular cells, thyroid follicular cells), usually showing a weaker, cytoplasmic reaction. CD117 is also demonstrated in testicular and ovarian interstitial cells, in neurons of the central nervous system (cerebellum, hippocampus, and dorsal horn of the spinal cord), and in immature myeloid cells. CD117 does not occur in smooth muscle cells. Family of transmembrane proteins essential for the regulation of cell growth and maintenance. When a growth factor is bound to its receptor, the latter is phosphorylated and begins a cascade of intracytoplasmic signals. Cells involved in the generation of electrical pacemaker activity for gastrointestinal motility. The cells are considered to be the origin of gastrointestinal stromal tumours. CD117 is of great importance for the classification of mesenchymal tumours of the gastrointestinal tract (including the mesentery). CD117 may also be used for classification of germinal cell tumours, as seminoma/dysgerminoma stains in the majority of cases, showing a strong membranous staining, while embryonal carcinoma stains in a small proportion of cases, with a weaker, membranous reaction. An panel for distinguishing seminoma from embryonal carcinoma should include CD30. Also in the identification of mast cell neoplasms CD117 has a potential. Appendix is recommended as positive and negative tissue controls for CD117.

Background References

1. Harris KS. et. al. CD117/c-kit defines a prostate CSC-like subpopulation driving progression and TKI resistance. Sci Rep. 2021 Jan

2. Russkamp NF. et. al. Anti-CD117 immunotherapy to eliminate hematopoietic and leukemia stem cells. Exp Hematol. 2021 Mar

Subcellular Location

Cell membrane; Cytoplasm.

UNIPROT

SwissProt: P10721 Human

Synonyms

C Kit antibody

c-Kit antibody

c-Kit Ligand antibody

CD117 antibody

Kit antibody

Kit Ligand antibody

KIT oncogene antibody

KIT proto oncogene receptor tyrosine kinase antibody

KIT_HUMAN antibody

Mast cell growth factor receptor antibody

Expand

Images

  • mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-c-Kit antibody (<a href="/products/IRS013" style="font-weight: bold;text-decoration: underline;">IRS013</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-c-Kit antibody (IRS013) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-c-Kit antibody (<a href="/products/IRS013" style="font-weight: bold;text-decoration: underline;">IRS013</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-c-Kit antibody (IRS013) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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