Skip to content

ICAM1 Recombinant Rabbit Monoclonal Antibody [ST0487]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1609-46

ICAM1 Recombinant Rabbit Monoclonal Antibody [ST0487]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ICAM1 Recombinant Rabbit Monoclonal Antibody [ST0487]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human ICAM1 aa 30-70.

Species Reactivity

Human

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 58 kDa

Positive Control

HepG2 cell lysate, Ramos cell lysate, Raji cell lysate, human liver tissue lysate, human colon cancer tissue, human lung cancer tissue, human kidney tissue, human lymph node tissue.

Conjugation

unconjugated

Clone Number

ST0487

RRID

AB_3069856

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200

Target

Function

Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play important, yet separate, roles in embryogenesis and development. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the beta2alpha1 and beta2alphaM Integrins. ICAM-2 functions as a ligand for lymphocyte function-associated antigen-1 (LFA-1) and is involved in leukocyte adhesion. ICAM-3 is highly expressed on the surface of human eosinophils and, when bound to ligand, may inhibit eosinophil inflammatory responses and survival. ICAM-4, also known as LW glycoprotein, interacts with Integrins alphaLbeta2, alphaMbeta2, alpha4beta1, the alphaV family and alphaIIbbeta3, and selective binding to different integrins may be relevant to the pathology in a number of red blood cell associated diseases. Lastly, ICAM-5, expressed on telencephalic neurons, binds CD11a/CD18 and thus may act as an adhesion molecule for leukocyte binding in the central nervous system.

Background References

1. Grover JR et al. Basic motifs target PSGL-1, CD43, and CD44 to plasma membrane sites where HIV-1 assembles. J Virol 89:454-67 (2015).

2. Rouault C et al. Roles of chemokine ligand-2 (CXCL2) and neutrophils in influencing endothelial cell function and inflammation of human adipose tissue. Endocrinology 154:1069-79 (2013).

Sequence Similarity

Belongs to the immunoglobulin superfamily. ICAM family.

Post-translational Modification

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P05362 Human

Synonyms

Antigen identified by monoclonal antibody

BB2 antibody

BB 2 antibody

BB2 antibody

CD 54 antibody

CD_antigen=CD54 antibody

CD54 antibody

Cell surface glycoprotein P3.58 antibody

Human rhinovirus receptor antibody

ICAM 1 antibody

Expand

Images

  • Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: Ramos cell lysate (20 µg/Lane)<br />Lane 3: Raji cell lysate (20 µg/Lane)<br />Lane 4: human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 58 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: Ramos cell lysate (20 µg/Lane)
    Lane 3: Raji cell lysate (20 µg/Lane)
    Lane 4: human liver tissue lysate (40 µg/Lane)

    Predicted band size: 58 kDa
    Observed band size: 110 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: K-562 cell lysate (low expression)<br />Lane 3: Raji cell lysate<br />Lane 4: Ramos cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: K-562 cell lysate (low expression)
    Lane 3: Raji cell lysate
    Lane 4: Ramos cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 110 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-46" style="font-weight: bold;text-decoration: underline;">ET1609-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody (ET1609-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

ICAM1 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

ICAM1 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

ICAM1 Recombinant Rabbit Monoclonal Antibody [ST0487] - BSA and Azide free

Application: WB,IF-Tissue,IHC-P

Reactivity: Human

Conjugate: unconjugated

metafield image

ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH09-31]

Application: WB,IHC-P,IF-Tissue

Reactivity: Human

Conjugate: unconjugated

metafield image

HRP Conjugated Human ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH10-16] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: HRP

metafield image

Human ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH10-15] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human

Conjugate: unconjugated

metafield image

Biotin Conjugated Human ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH10-16] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human

Conjugate: Biotin

metafield image

ICAM1 Mouse Monoclonal Antibody [F11-A4]

Application: WB,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

ICAM1 Mouse Monoclonal Antibody [J0-F7]

Application: WB,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Human ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH10-16] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human

Conjugate: unconjugated