IFNAR1 Mouse Monoclonal Antibody [A5-A3]
Catalog# EM1701-46
IFNAR1 Mouse Monoclonal Antibody [A5-A3]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
IFNAR1 Mouse Monoclonal Antibody [A5-A3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human IFNAR1 aa 28-127 (Extracellular).
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 64 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, NCCIT, SHG-44, human lung tissue, human colon tissue, human skin tissue.
Conjugation
unconjugated
Clone Number
A5-A3
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Interferon-alpha/beta receptor alpha chain is a protein that in humans is encoded by the IFNAR1 gene.
Background References
1. Thomas C et al. Structural linkage between ligand discrimination and receptor activation by type I interferons. Cell 146:621-632 (2011).
2. Claudinon J et al. Palmitoylation of interferon-alpha (IFN-alpha) receptor subunit IFNAR1 is required for the activation of Stat1 and Stat2 by IFN-alpha. J. Biol. Chem. 284:24328-24340 (2009).
Sequence Similarity
Belongs to the type II cytokine receptor family.
Tissue Specificity
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
Post-translational Modification
Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex.; Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination. Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Phosphorylated on tyrosine residues in response to interferon.; Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.
Subcellular Location
Endosome. Cell membrane.
Synonyms
Alpha type antiviral protein antibody
Antiviral protein, alpha-type antibody
Antiviral protein, beta-type antibody
AVP antibody
Beta type antiviral protein antibody
CRF2-1 antibody
Cytokine receptor class-II member 1 antibody
Cytokine receptor family 2 member 1 antibody
IFN alpha REC antibody
IFN alpha receptor antibody
ExpandImages
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Western blot analysis of IFNAR1 on different lysates with Mouse anti-IFNAR1 antibody (EM1701-46) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64/90/130 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-46) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. -
ICC staining IFNAR1(red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining IFNAR1(green) in SHG-44 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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P-selectin overexpression impairs hematopoietic stem cell homeostasis via inflammatory receptor-mediated proliferation and differentiation
Journal: Cell Death & Disease
DOI: 10.1038/s41419-025-08050-9
IF: 9.6
Application: IF-cell
Reactivity: Mouse
Publish date: 2025 Oct
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Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IFNAR1 Recombinant Rabbit Monoclonal Antibody [SR45-08] - BSA and Azide free
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
