IFNAR1 Rabbit Polyclonal Antibody
Catalog# R1511-30
IFNAR1 Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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FC
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
IFNAR1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human IFNAR1 aa 28-227/557.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P, FC, IF-Tissue
Molecular Weight
Predicted band size: 64 kDa
Positive Control
Human liver tissue, SW620, K562.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IF-Tissue
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1:500
Target
Function
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.
Background References
1. Blocking IFNAR1 inhibits multiple myeloma-driven Treg expansion and immunosuppression. Kawano Y. et. al. J Clin Invest. 2018 Jun
2. Expression of IFNAR1 and IFNAR2 in cattle placenta during early pregnancy. Wang W. et. al. Reprod Domest Anim. 2018 Apr
Sequence Similarity
Belongs to the type II cytokine receptor family.
Tissue Specificity
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
Post-translational Modification
Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex.; Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination. Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Phosphorylated on tyrosine residues in response to interferon.; Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.
Subcellular Location
Late endosome, Lysosome, Cell membrane.
UNIPROT
Synonyms
Alpha type antiviral protein antibody
Antiviral protein, alpha-type antibody
Antiviral protein, beta-type antibody
AVP antibody
Beta type antiviral protein antibody
CRF2-1 antibody
Cytokine receptor class-II member 1 antibody
Cytokine receptor family 2 member 1 antibody
IFN alpha REC antibody
IFN alpha receptor antibody
ExpandImages
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Application: IF-Tissue
Species: Human
Site: liver
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IFNAR1 antibody (R1511-30) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-30) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
ICC staining of IFNAR1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1511-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of IFNAR1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (R1511-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
IFNAR1 Recombinant Rabbit Monoclonal Antibody [SR45-08]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
IFNAR1 Recombinant Rabbit Monoclonal Antibody [SR45-08]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IFNAR1 Mouse Monoclonal Antibody [A5-A3]
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse
Conjugate: unconjugated
