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IL-10 Rabbit Polyclonal Antibody

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Catalog# ER1911-19

IL-10 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IL-10 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from human IL-10 101-178/178

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 21 kDa

Positive Control

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

Target

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.

Background References

暂无

Subcellular Location

Secreted.

UNIPROT

SwissProt: P22301 Human

SwissProt: P18893 Mouse

SwissProt: P29456 Rat

Synonyms

CSIF antibody

Cytokine synthesis inhibitory factor antibody

GVHDS antibody

IL 10 antibody

IL-10 antibody

IL10 antibody

IL10_HUMAN antibody

IL10A antibody

Interleukin 10 antibody

Interleukin-10 antibody

Expand

Images

  • Sample: <br />Lane 1: Recombinant human IL-10 protein, His (HEK293) <br />Primary: Anti-IL-10 (<a href="/products/ER1911-19" style="font-weight: bold;text-decoration: underline;">ER1911-19</a>) at 1/1000 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 18 kDa<br />Observed band size: 21 kDa

    Sample:
    Lane 1: Recombinant human IL-10 protein, His (HEK293)
    Primary: Anti-IL-10 (ER1911-19) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 18 kDa
    Observed band size: 21 kDa

  • Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(<a href="/products/ER1911-19" style="font-weight: bold;text-decoration: underline;">ER1911-19</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(ER1911-19) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (<a href="/products/ER1911-19" style="font-weight: bold;text-decoration: underline;">ER1911-19</a>) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining

    Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (ER1911-19) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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