IL-10RB Rabbit Polyclonal Antibody

Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA500322) at 1/500 dilution.

Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 37 kDa
Observed band size: 42 kDa (Glycoprotein)

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500322) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SiHa cells labeling IL-10RB with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow cytometric analysis of THP-1 cells labeling IL-10RB.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500322, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).