IL-10RB Mouse Monoclonal Antibody [A8E11]
Catalog# HA601061
IL-10RB Mouse Monoclonal Antibody [A8E11]
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WB
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Human
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unconjugated
Overview
Product Name
IL-10RB Mouse Monoclonal Antibody [A8E11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human IL-10RB aa 181-230 / 325.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 37 kDa
Positive Control
Jurkat cell lysate, 293T cell lysate, U-87 MG cell lysate, THP-1 cell lysate.
Conjugation
unconjugated
Clone Number
A8E11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Interleukin 10 receptor, beta subunit is a subunit for the interleukin-10 receptor. IL10RB is its human gene. IL10RB has also recently been designated CDW210B (cluster of differentiation W210B). The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFNAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.
Background References
1. Voloudakis G et al. IL10RB as a key regulator of COVID-19 host susceptibility and severity. medRxiv. 2021 Jun
2. Yazdani R et al. Candidiasis associated with very early onset inflammatory bowel disease: First IL10RB deficient case from the National Iranian Registry and review of the literature. Clin Immunol. 2019 Aug
Subcellular Location
Membrane.
UNIPROT
Synonyms
Cd210 antibody
CDw210b antibody
CRF2 4 antibody
CRF2-4 antibody
CRFB4 antibody
Cytokine receptor class II antibody
Cytokine receptor class II CRF2 4 antibody
Cytokine receptor class-II member 4 antibody
Cytokine receptor family 2 member 4 antibody
Cytokine receptor family II member 4 antibody
ExpandImages
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Western blot analysis of IL-10RB on different lysates with Mouse anti-IL-10RB antibody (HA601061) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 42 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601061) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Expression of the readthrough transcript CiDRE in alveolar macrophages boosts SARS-CoV-2 susceptibility and promotes COVID-19 severity
Journal: Immunity
DOI: 10.1016/j.immuni.2023.06.013
IF: 32.4
Application: WB
Reactivity: Human
Publish date: 2023 Jul
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